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1
Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14α-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B.酿酒酵母 ERG11/CYP51（甾醇 14α-脱甲基酶）强力霉素调控突变体的互补作用和烟曲霉同工酶 CYP51A 和 CYP51B 的唑类敏感性筛选。
Antimicrob Agents Chemother. 2010 Nov;54(11):4920-3. doi: 10.1128/AAC.00349-10. Epub 2010 Aug 23.
2
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3
Expression, purification, and characterization of Aspergillus fumigatus sterol 14-alpha demethylase (CYP51) isoenzymes A and B.烟曲霉甾醇 14-α 脱甲基酶（CYP51）同工酶 A 和 B 的表达、纯化和特性。
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4
Facultative sterol uptake in an ergosterol-deficient clinical isolate of Candida glabrata harboring a missense mutation in ERG11 and exhibiting cross-resistance to azoles and amphotericin B.具有 ERG11 错义突变并对唑类和两性霉素 B 表现交叉耐药的光滑假丝酵母临床分离株中的兼性甾醇摄取。
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In Vitro Biochemical Study of CYP51-Mediated Azole Resistance in Aspergillus fumigatus.烟曲霉中CYP51介导的唑类耐药性的体外生化研究
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Targeted gene disruption of the 14-alpha sterol demethylase (cyp51A) in Aspergillus fumigatus and its role in azole drug susceptibility.烟曲霉中14-α-甾醇去甲基化酶（cyp51A）的靶向基因破坏及其在唑类药物敏感性中的作用。
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Impact of Homologous Resistance Mutations from Pathogenic Yeast on Saccharomyces cerevisiae Lanosterol 14α-Demethylase.致病酵母同源抗性突变对酿酒酵母麦角甾醇 14α-脱甲基酶的影响。
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Molecular Mechanisms of Antifungal Resistance in Mucormycosis.黏液性毛霉病中抗真菌耐药的分子机制。
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本文引用的文献

1
New resistance mechanisms to azole drugs in Aspergillus fumigatus and emergence of antifungal drugs-resistant A. fumigatus atypical strains.烟曲霉对唑类药物的新耐药机制及耐唑类药物烟曲霉非典型菌株的出现。
Med Mycol. 2006 Sep 1;44(Supplement_1):S367-S371. doi: 10.1080/13693780600902243.
2
Expression, purification, and characterization of Aspergillus fumigatus sterol 14-alpha demethylase (CYP51) isoenzymes A and B.烟曲霉甾醇 14-α 脱甲基酶（CYP51）同工酶 A 和 B 的表达、纯化和特性。
Antimicrob Agents Chemother. 2010 Oct;54(10):4225-34. doi: 10.1128/AAC.00316-10. Epub 2010 Jul 26.
3
A clinical isolate of Candida albicans with mutations in ERG11 (encoding sterol 14alpha-demethylase) and ERG5 (encoding C22 desaturase) is cross resistant to azoles and amphotericin B.一株具有 ERG11（编码甾醇 14α-脱甲基酶）和 ERG5（编码 C22 去饱和酶）基因突变的白念珠菌临床分离株对唑类药物和两性霉素 B 交叉耐药。
Antimicrob Agents Chemother. 2010 Sep;54(9):3578-83. doi: 10.1128/AAC.00303-10. Epub 2010 Jun 14.
4
Heterologous expression of mutated eburicol 14alpha-demethylase (CYP51) proteins of Mycosphaerella graminicola to assess effects on azole fungicide sensitivity and intrinsic protein function.异源表达球腔菌突变的麦角甾醇 14α-脱甲基酶（CYP51）蛋白以评估其对唑类杀菌剂敏感性和固有蛋白功能的影响。
Appl Environ Microbiol. 2010 May;76(9):2866-72. doi: 10.1128/AEM.02158-09. Epub 2010 Mar 19.
5
Azole resistance in Aspergillus fumigatus: a side-effect of environmental fungicide use?烟曲霉中的唑类抗性：环境中使用杀菌剂的副作用？
Lancet Infect Dis. 2009 Dec;9(12):789-95. doi: 10.1016/S1473-3099(09)70265-8.
6
Efficacy of posaconazole against three clinical Aspergillus fumigatus isolates with mutations in the cyp51A gene.泊沙康唑对 3 株 CYP51A 基因突变的临床烟曲霉分离株的疗效。
Antimicrob Agents Chemother. 2010 Feb;54(2):860-5. doi: 10.1128/AAC.00931-09. Epub 2009 Nov 16.
7
Trends in invasive fungal infections, with emphasis on invasive aspergillosis.侵袭性真菌感染的趋势，重点是侵袭性曲霉病。
Clin Microbiol Infect. 2009 Jul;15(7):625-33. doi: 10.1111/j.1469-0691.2009.02929.x.
8
Frequency and evolution of Azole resistance in Aspergillus fumigatus associated with treatment failure.烟曲霉中唑类耐药与治疗失败相关的频率及演变
Emerg Infect Dis. 2009 Jul;15(7):1068-76. doi: 10.3201/eid1507.090043.
9
Possible environmental origin of resistance of Aspergillus fumigatus to medical triazoles.烟曲霉对医用三唑类药物耐药性可能的环境起源
Appl Environ Microbiol. 2009 Jun;75(12):4053-7. doi: 10.1128/AEM.00231-09. Epub 2009 Apr 17.
10
Avian Aspergillus fumigatus strains resistant to both itraconazole and voriconazole.对伊曲康唑和伏立康唑均耐药的禽烟曲霉菌株。
Antimicrob Agents Chemother. 2009 May;53(5):2199-201. doi: 10.1128/AAC.01492-08. Epub 2009 Mar 2.

酿酒酵母 ERG11/CYP51（甾醇 14α-脱甲基酶）强力霉素调控突变体的互补作用和烟曲霉同工酶 CYP51A 和 CYP51B 的唑类敏感性筛选。

Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14α-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B.

机构信息

Institute of Life Science and School of Medicine, Swansea University, Swansea, Wales, United Kingdom.

出版信息

Antimicrob Agents Chemother. 2010 Nov;54(11):4920-3. doi: 10.1128/AAC.00349-10. Epub 2010 Aug 23.

DOI:10.1128/AAC.00349-10
PMID:20733045
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2976139/
Abstract

Aspergillus fumigatus sterol 14α-demethylase isoenzymes CYP51A and CYP51B were heterologously expressed in a Saccharomyces cerevisiae mutant (YUG37-erg11), wherein native ERG11/CYP51 expression is controlled using a doxycycline-regulatable promoter. When cultured in the presence of doxycycline, recombinant YUG37-pcyp51A and YUG37-pcyp51B yeasts were able to synthesize ergosterol and grow; a control strain harboring reverse-oriented cyp51A could not. YUG37-pcyp51A and YUG37-pcyp51B constructs showed identical sensitivity to itraconazole, posaconazole, clotrimazole, and voriconazole. Conversely, YUG37-pcyp51A withstood 16-fold-higher concentrations of fluconazole than YUG37-pcyp51B (8 and 0.5 μg ml⁻¹, respectively).

摘要

烟曲霉甾醇 14α-脱甲基酶同工酶 CYP51A 和 CYP51B 在酿酒酵母突变体（YUG37-erg11）中异源表达，其中天然 ERG11/CYP51 的表达受强力霉素可调控启动子控制。当在强力霉素存在下培养时，重组 YUG37-pcyp51A 和 YUG37-pcyp51B 酵母能够合成麦角固醇并生长；而含有反向定向 cyp51A 的对照菌株则不能。YUG37-pcyp51A 和 YUG37-pcyp51B 构建体对伊曲康唑、泊沙康唑、克霉唑和伏立康唑的敏感性相同。相反，YUG37-pcyp51A 能耐受比 YUG37-pcyp51B 高 16 倍的氟康唑浓度（分别为 8 和 0.5 μg ml⁻¹）。