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硅酮化合物改变了甲壳素胶和模型酶固化过程中的酶活性。

Compounds from silicones alter enzyme activity in curing barnacle glue and model enzymes.

机构信息

MSC Division, Duke University Marine Laboratory, Nicholas School of the Environment, Beaufort, North Carolina, United States of America.

出版信息

PLoS One. 2011 Feb 17;6(2):e16487. doi: 10.1371/journal.pone.0016487.

DOI:10.1371/journal.pone.0016487
PMID:21379573
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3040736/
Abstract

BACKGROUND

Attachment strength of fouling organisms on silicone coatings is low. We hypothesized that low attachment strength on silicones is, in part, due to the interaction of surface available components with natural glues. Components could alter curing of glues through bulk changes or specifically through altered enzyme activity.

METHODOLOGY/PRINCIPAL FINDINGS: GC-MS analysis of silicone coatings showed surface-available siloxanes when the coatings were gently rubbed with a cotton swab for 15 seconds or given a 30 second rinse with methanol. Mixtures of compounds were found on 2 commercial and 8 model silicone coatings. The hypothesis that silicone components alter glue curing enzymes was tested with curing barnacle glue and with commercial enzymes. In our model, barnacle glue curing involves trypsin-like serine protease(s), which activate enzymes and structural proteins, and a transglutaminase which cross-links glue proteins. Transglutaminase activity was significantly altered upon exposure of curing glue from individual barnacles to silicone eluates. Activity of purified trypsin and, to a greater extent, transglutaminase was significantly altered by relevant concentrations of silicone polymer constituents.

CONCLUSIONS/SIGNIFICANCE: Surface-associated silicone compounds can disrupt glue curing and alter enzyme properties. Altered curing of natural glues has potential in fouling management.

摘要

背景

附着在硅酮涂层上的污垢生物的附着强度较低。我们假设硅酮上的低附着强度部分是由于表面可用成分与天然胶水的相互作用。这些成分可能会通过整体变化或通过改变酶活性来改变胶水的固化。

方法/主要发现：用棉签轻轻擦拭 15 秒或用甲醇冲洗 30 秒后，对硅酮涂层进行 GC-MS 分析，显示出表面可用的硅氧烷。在 2 种商业和 8 种模型硅酮涂层上发现了混合物。用固化藤壶胶和商业酶测试了硅酮成分改变胶水固化酶的假设。在我们的模型中，藤壶胶的固化涉及胰蛋白酶样丝氨酸蛋白酶（trypsin-like serine protease，s），它激活酶和结构蛋白，以及一种转谷氨酰胺酶（transglutaminase），它交联胶水蛋白。在用硅胶洗脱液暴露来自单个藤壶的固化胶时，转谷氨酰胺酶活性明显改变。纯化的胰蛋白酶活性，以及更显著的转谷氨酰胺酶活性，都被相关浓度的硅酮聚合物成分显著改变。

结论/意义：表面相关的硅酮化合物可以破坏胶水的固化并改变酶的性质。天然胶水固化的改变在防污管理中有潜在应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/b572bc6fb240/pone.0016487.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/10175d0bf21a/pone.0016487.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/6b91b5d8f22f/pone.0016487.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/34ac111b688f/pone.0016487.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/8e6cfee03717/pone.0016487.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/144b36c4386c/pone.0016487.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/3484087bdc99/pone.0016487.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/b572bc6fb240/pone.0016487.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/10175d0bf21a/pone.0016487.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/6b91b5d8f22f/pone.0016487.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/34ac111b688f/pone.0016487.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/8e6cfee03717/pone.0016487.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/144b36c4386c/pone.0016487.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/3484087bdc99/pone.0016487.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa4/3040736/b572bc6fb240/pone.0016487.g007.jpg

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