Department of Chemistry and Chemical Biology, McMaster University, Hamilton, ON, L8S 4M1, Canada.
Eur Biophys J. 2011 Jun;40(6):805-10. doi: 10.1007/s00249-011-0690-7. Epub 2011 Mar 5.
The interaction of antimicrobial peptide anoplin with 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] lipid monolayers was imaged with atomic force microscopy, scanning transmission X-ray microscopy, and X-ray photoemission electron microscopy. X-ray absorption spectromicroscopy of the surface revealed the domains of the phase-segregated surface to be composed of 98(±5)% lipid while the matrix consisted of a ~50:50 lipid-peptide mixture. We show X-ray spectromicroscopy to be a valuable quantitative tool for label-free imaging of lipid monolayers with antimicrobial peptides at a lateral spatial resolution below 80 nm.
采用原子力显微镜、扫描透射 X 射线显微镜和 X 射线光电子能谱显微镜对抗菌肽 anoplin 与 1,2-二棕榈酰基-sn-甘油-3-[磷酸-rac-(1-甘油)]脂质单层的相互作用进行了成像。表面的 X 射线吸收光谱显微镜显示，相分离表面的畴由 98(±5)%的脂质组成，而基质由 ~50:50 的脂质-肽混合物组成。我们表明，X 射线光谱显微镜是一种有价值的定量工具，可用于在 80nm 以下的横向空间分辨率下对带有抗菌肽的脂质单层进行无标记成像。
