Reuter R, Rothe S, Habets W, Van Venrooij W J, Lührmann R
Max-Planck-Institut für molekulare Genetik, Otto-Warburg-Laboratorium, Berlin, FRG.
Eur J Immunol. 1990 Feb;20(2):437-40. doi: 10.1002/eji.1830200231.
The nucleoplasmic U small nuclear ribonucleoprotein particles (snRNP) have a set of seven proteins in common which are designated B', B, D, D', E, F and G. Patients suffering from rheumatoid autoimmune diseases such as systemic lupus erythematosus often develop autoantibodies against the proteins B', B, and D. Here we describe a sensitive immunoassay which allows the specific detection of autoantibodies reacting with the E, F or G snRNP proteins. We were able to identify several patient sera containing autoantibodies against one or more of these proteins. This demonstrates that all snRNP proteins described so far are potentially antigenic in systemic rheumatoid diseases. The characterization of the antibodies showed an immunological cross-reactivity between the snRNP protein G and the 70-kDa protein of U1 snRNP. Several sera contained autoantibodies which were specific for the F snRNP protein.
核质U小核核糖核蛋白颗粒（snRNP）有一组共七种常见蛋白质，分别命名为B'、B、D、D'、E、F和G。患有类风湿性自身免疫疾病（如系统性红斑狼疮）的患者常常会产生针对蛋白质B'、B和D的自身抗体。在此，我们描述了一种灵敏的免疫测定法，可特异性检测与E、F或G snRNP蛋白质发生反应的自身抗体。我们能够鉴定出几份含有针对这些蛋白质中一种或多种的自身抗体的患者血清。这表明，迄今为止所描述的所有snRNP蛋白质在系统性类风湿疾病中都可能具有抗原性。抗体的特性表明，snRNP蛋白质G与U1 snRNP的70 kDa蛋白质之间存在免疫交叉反应。几份血清含有对F snRNP蛋白质具有特异性的自身抗体。
