Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr University Bochum, Bürkle-de-la-Camp Platz 1, 44789 Bochum, Germany.
J Orthop Res. 2011 Aug;29(8):1237-44. doi: 10.1002/jor.21358. Epub 2011 Mar 4.
Sufficient induction of blood vessel ingrowth decisively influence transplant functionality. In this study, microvascular response to transplants of surface modified bone substitutes were assessed in vivo. The surface modification of allogenic bone substitutes (dehydrated human femoral head) was achieved in a double-conductive low-pressure gasplasma reactor (Ar(2) /O(2) , 13.65 MHz, 1,000 W, 5 Pa). The modified bone substitutes (n = 10) as well as untreated bone substitutes serving as controls (n = 10) were placed into the dorsal skinfold chamber of female balb/c mice (n = 10). Dynamic assessment of microcirculatory parameters was performed using intravital fluorescence microscopy during an implantation period of 10 days. The angiogenic response was found markedly accelerated in gasplasma-treated bone. Compared to untreated implants, the gasplasma-activated bone substitutes showed significantly higher microvascular density on days 5 and 10. The quantification of the microvascular diameters, red blood cell velocity, and microvascular permeability displayed stable perfusion and vascular integrity of the newly developed blood vessels throughout the 10-day observation period. The surface activation via cold low-pressure glow discharge gasplasma supports the vascular integration of allogenic bone by earlier induction of the angiogenesis.
足够的血管生成诱导对移植物的功能有决定性影响。在这项研究中，评估了表面改性骨替代物的体内微血管反应。通过双导电低压气体等离子体反应器（Ar(2) / O(2)，13.65MHz，1000W，5Pa）对同种异体骨替代物（脱水人股骨头）进行表面改性。将改性骨替代物（n=10）和未处理的作为对照的骨替代物（n=10）放置在雌性 Balb/c 小鼠的背部皮褶室中（n=10）。在植入期的 10 天内，通过活体荧光显微镜进行微循环参数的动态评估。结果发现，气体等离子体处理的骨的血管生成反应明显加快。与未处理的植入物相比，气体等离子体激活的骨替代物在第 5 天和第 10 天的微血管密度显著更高。微血管直径、红细胞速度和微血管通透性的定量显示，在 10 天的观察期内，新形成的血管的灌注和血管完整性稳定。通过冷低压辉光放电气体等离子体进行的表面激活通过更早地诱导血管生成来支持同种异体骨的血管整合。
