Chirurgische Klinik und Poliklinik, Berufsgenossenschaftliches Universitätsklinikum Bergmannsheil GmbH, Bürkle-de-la-Camp Platz 1, 44795, Bochum, Germany.
Langenbecks Arch Surg. 2010 Nov;395(8):1147-55. doi: 10.1007/s00423-010-0608-3. Epub 2010 Mar 7.
The purpose was to evaluate inflammatory and microcirculatory reactions after implantation of various calcium phosphate bone substitutes in an in vivo model.
Calcium phosphate-based bone substitutes were implanted in dorsal skinfold chambers of mice. Intravital fluorescence microscopy was performed to measure inflammatory and microcirculatory reactions based on functional vessel density (FVD), capillary leakage, and relative white blood cell velocity (rWBCV).
An increase of FVD was observed in all groups and the capillary leakage grew with a level of significance (p < 0.001). The fraction of rolling and sticking leukocytes (rWBCV) was highest at the beginning of the trial and decreased during the course.
There are differences in microvascular soft tissue reactions between various calcium phosphate bone substitutes, but inflammatory reactions were moderate, and the results revealed no reasons which explain the sporadic failure of the tested substances under clinical conditions.
评估不同钙磷骨替代物植入体内模型后的炎症和微循环反应。
将钙磷基骨替代物植入小鼠背部皮褶室。通过活体荧光显微镜测量炎症和微循环反应，基于功能血管密度（FVD）、毛细血管渗漏和相对白细胞速度（rWBCV）。
所有组均观察到 FVD 增加，毛细血管渗漏具有显著性增加（p < 0.001）。滚动和黏附白细胞的分数（rWBCV）在试验开始时最高，随后逐渐下降。
各种钙磷骨替代物的软组织微血管反应存在差异，但炎症反应适中，结果未发现可解释受试物质在临床条件下偶发失败的原因。
