School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, PR China.
Biotechnol J. 2011 May;6(5):492-500. doi: 10.1002/biot.201000395. Epub 2011 Mar 7.
A simple and relatively cheap glucose biosensor based on a combination of gold nanoparticles (Au NPs) and glucose oxidase (GO(x) ) immobilized on a bioplatform eggshell membrane was established. Scanning electron microscopy showed successful immobilization of Au NPs/GO(x) on the eggshell membrane. The effects of pH, phosphate buffer concentration, and temperature on the glucose biosensor were studied in detail. The biosensor shows a linear response at a glucose concentration range of 5-525 μM. The detection limit of the biosensor is 2.5 μM (S/N = 3). The biosensor exhibits good repeatability with RSD = 3.6% (n = 6), good operational stability with over 300 measurements and long-term storage stability with a shelf life of at least 6 months. The response time is less than 60 s. The glucose level in commercial food samples has been successfully determined. The proposed work shows potential to develop cost-effective biosensors for biotechnological, biomedical and industrial use.
基于金纳米粒子（Au NPs）和固定在生物平台蛋壳膜上的葡萄糖氧化酶（GO(x)）的组合，建立了一种简单且相对廉价的葡萄糖生物传感器。扫描电子显微镜显示 Au NPs/GO(x)成功固定在蛋壳膜上。详细研究了 pH、磷酸盐缓冲液浓度和温度对葡萄糖生物传感器的影响。该生物传感器在葡萄糖浓度为 5-525 μM 的范围内呈线性响应。生物传感器的检测限为 2.5 μM（S/N = 3）。该生物传感器具有良好的重复性，RSD = 3.6%（n = 6），良好的操作稳定性，超过 300 次测量，以及至少 6 个月的长期储存稳定性。响应时间小于 60 秒。已成功测定商业食品样品中的葡萄糖含量。该研究工作表明，有可能开发用于生物技术、生物医学和工业用途的具有成本效益的生物传感器。
