Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook Facility, Rockville, MD, USA.
Wiley Interdiscip Rev Syst Biol Med. 2011 Nov-Dec;3(6):717-27. doi: 10.1002/wsbm.143. Epub 2011 Mar 4.
Dictyostelium discoideum has been chosen as the key model organism for the study of eukaryotic chemotaxis. Studies in this lower eukaryotic organism have allowed us to discover eukaryotic chemotaxis behavior and to gradually understand the mechanism of chemotaxis. Investigations in this simple organism often guide the direction of chemotaxis studies in areas such as forming concepts, discovering molecular components, revealing pathways and networks. The cooperation between experimental approaches and computational modeling has helped us to comprehend the signaling network as a system. To further reveal the relationships among the molecular mechanisms of individual signaling steps, a continuous interplay between model development and refinement and experimental testing and verification will be useful. This article focuses on a chemoattractant G-protein-coupled receptor (GPCR)/G-protein gradient sensing machinery, which is monitored by PIP(3) responses and investigated by the interplay between live cell imaging experiments and computational modeling. We believe that such an approach will lead to a much better understanding of GPCR-controlled chemotaxis of all eukaryotic cells.
粘菌（Dictyostelium discoideum）已被选为研究真核趋化性的关键模式生物。在这个较低等真核生物中的研究使我们能够发现真核趋化性行为，并逐渐了解趋化性的机制。在这个简单生物中的研究通常为趋化性研究的各个领域（如概念形成、发现分子成分、揭示途径和网络）提供指导方向。实验方法和计算模型之间的合作有助于我们将信号网络理解为一个系统。为了进一步揭示单个信号步骤的分子机制之间的关系，模型开发和细化以及实验测试和验证之间的持续相互作用将是有用的。本文重点介绍了由 PIP(3) 反应监测的趋化性 G 蛋白偶联受体 (GPCR)/G 蛋白梯度感应机制，并通过活细胞成像实验和计算模型之间的相互作用进行了研究。我们相信，这种方法将有助于更好地理解所有真核细胞的 GPCR 控制的趋化性。
