Romaniuk L V, Tovkach F I, Ivanitsa T V, Kushkina A I, Ostapchuk A N, Gorb T E
Mikrobiol Z. 2010 Nov-Dec;72(6):51-7.
A possibility to obtain nanoparticles of phage nature using abortive phage infection was shown for the first time. It was found out that the nonspecific host Erwinia carotovora subsp. carotovora J2 being infected by phage ZF 40-RT80, the cells form a 100-fold surplus of capsid structures. Using the electron microscope the authors have found two types of phage capsids which differ from each other and have different modal diameters--47.5 and 71.5 nm. The found capsids pack the phage DNA which releases them under treatment of the preparations by DNAse I. A simple method of purification of capsid structures from mature virions which are formed in inconsiderable quantity in the process of abortive phage infection is proposed. The obtained results create preconditions for obtaining capsid nanoparticles as well as for studying the stages of morphogenesis and morphopoiesis of phage ZF40 without attracting special phage mutants.
首次展示了利用流产噬菌体感染获得具有噬菌体性质的纳米颗粒的可能性。研究发现，非特异性宿主胡萝卜软腐欧文氏菌胡萝卜软腐亚种J2在被噬菌体ZF 40-RT80感染时，细胞会形成比衣壳结构多100倍的物质。作者利用电子显微镜发现了两种彼此不同且具有不同模态直径（47.5和71.5纳米）的噬菌体衣壳。所发现的衣壳包裹着噬菌体DNA，在制备物经DNA酶I处理后会释放出这些DNA。提出了一种从流产噬菌体感染过程中少量形成的成熟病毒粒子中纯化衣壳结构的简单方法。所获得的结果为获得衣壳纳米颗粒以及研究噬菌体ZF40的形态发生和形态建成阶段创造了前提条件，而无需引入特殊的噬菌体突变体。
