Kiseleva I V, Larionova N V, Voeten J T M, Teley L C P, Drieszen-van der Cruijsen S K M, Heldens J G M, van den Bosch J F, Rudenko L G
Zh Mikrobiol Epidemiol Immunobiol. 2010 Nov-Dec(6):41-7.
To identify the genes that are responsible for attenuation of donor viruses for live influenza vaccine. MATERIALS AND METHODS. Analysis of phenotypical properties of reassortants of wild type A and B influenza viruses with A/Leningrad/134/17/57 (H2N2) (A17) and B/USSR/60/69 (B60) master donor viruses was performed by comparison of their capability to grow at different temperatures in chicken eggs or/and MDCK cells.
Ts phenotype of 178 reassortants of A17 with current non-ts influenza A wild type viruses and 33 reassortants of B60 with current non-ts influenza B wild type viruses were evaluated. Reassortants inherited two polymerase genes PB2 and PA or PB 1 from A17 regularly demonstrated ts phenotype. The polymerase PA and PB2 gene segments of B60 independently controlled manifestation of ts phenotype of B60 based reassortants. The other nonpolymerase genes played no role in manifestation of ts phenotype of reassortants A17 and B60 viruses.
The molecular basis for the development ts phenotype of both A and B influenza vaccine reassortant viruses determined by polymerase genes complex.
鉴定负责减毒活流感疫苗供体病毒的基因。材料与方法。通过比较野生型甲型和乙型流感病毒与A/列宁格勒/134/17/57（H2N2）（A17）和B/苏联/60/69（B60）主供体病毒重配体在鸡胚或/和MDCK细胞中于不同温度下生长的能力，对其表型特性进行分析。
评估了178个A17与当前非温度敏感型甲型流感野生型病毒的重配体以及33个B60与当前非温度敏感型乙型流感野生型病毒的重配体的温度敏感（Ts）表型。从A17继承两个聚合酶基因PB2和PA或PB1的重配体通常表现出Ts表型。B60的聚合酶PA和PB2基因片段独立控制基于B60的重配体的Ts表型表现。其他非聚合酶基因在A17和B60病毒重配体的Ts表型表现中不起作用。
甲型和乙型流感疫苗重配体病毒温度敏感表型产生的分子基础由聚合酶基因复合体决定。
