Ortenwall P, Wadenvik H, Kutti J, Risberg B
Department of Surgery, University of Göteborg, Sweden.
J Vasc Surg. 1990 Mar;11(3):403-10.
Vascular prostheses in humans do not endothelialize spontaneously. In the present study we explored the feasibility of seeding autologous endothelial cells into prostheses implanted in patients undergoing reconstruction of the infrarenal aorta. In 22 patients one limb of an aortic Dacron bifurcation prosthesis was seeded with autologous endothelial cells harvested from the distal portion of the saphenous vein. The other limb was sham-seeded with culture medium only. The effect of seeding was studied by use of indium 111 radiolabeled platelets and external gamma camera scanning at 1, 4, and 12 months after surgery. No complications ascribable to the seeding procedure were seen. During the first year after surgery a gradual decrease in platelet accumulation occurred over the whole vascular prosthesis. At all time points studied the seeded graft limbs exhibited significantly less deposition of radiolabeled platelets than did control limbs. The observed difference in platelet accumulation on autologous endothelial seeding-treated graft segments merits further investigation of this technique in humans.
人体血管假体不会自发地进行内皮化。在本研究中，我们探讨了将自体内皮细胞接种到接受肾下腹主动脉重建术患者所植入假体中的可行性。在22例患者中，将取自大隐静脉远端的自体内皮细胞接种到主动脉涤纶分叉假体的一个分支上。另一个分支仅用培养基进行假接种。通过使用铟111放射性标记的血小板以及在术后1、4和12个月进行外部γ相机扫描来研究接种的效果。未观察到归因于接种程序的并发症。在术后第一年，整个血管假体上的血小板积聚逐渐减少。在所有研究的时间点，接种的移植分支上放射性标记血小板的沉积明显少于对照分支。在自体内皮接种处理的移植段上观察到的血小板积聚差异值得在人体中进一步研究该技术。
