Ortenwall P, Wadenvik H, Kutti J, Risberg B
J Vasc Surg. 1987 Jul;6(1):17-25. doi: 10.1067/mva.1987.avs0060017.
Autologous endothelial seeding (AES) of vascular prostheses in dogs increases thrombus-free surface and improves prosthetic prostacyclin production, patency, and the ability to withstand hematogenous challenge with bacteria. No such information is available in human subjects. In the present study one limb of an aortic Dacron bifurcation prosthesis was seeded with autologous endothelial cells (ECs) harvested from the distal portion of the saphenous vein by enzymatic treatment. The deposition of indium 111-labeled platelets on the vascular prostheses was studied 1 and 4 months after operation. In seven of nine patients seeding resulted in decreased accumulation of radiolabeled platelets compared with sham-seeded control limbs (p less than 0.04), when studied 1 month after surgery. A decrease in platelet accumulation occurred over the whole prosthesis between 1 and 4 months, and no significant difference was noted at 4 months between seeded and nonseeded graft limbs. Although the seeding density was very low (440 ECs/cm2), the observed difference in platelet accumulation for AES-treated graft limbs in the early postoperative course merits further investigation of this technique in human beings.
犬血管假体的自体内皮细胞种植（AES）可增加无血栓表面，并改善假体前列环素的产生、通畅性以及抵抗细菌血源性攻击的能力。在人类受试者中尚无此类信息。在本研究中，采用酶处理从大隐静脉远端采集的自体内皮细胞（ECs），种植于主动脉涤纶分叉假体的一个分支上。术后1个月和4个月研究了铟111标记血小板在血管假体上的沉积情况。术后1个月进行研究时，9例患者中有7例种植组与假种植对照组肢体相比，放射性标记血小板的积聚减少（p<0.04）。术后1至4个月期间，整个假体上的血小板积聚均减少，术后4个月时，种植组和未种植组移植肢体之间未观察到显著差异。尽管种植密度非常低（440个ECs/cm²），但术后早期AES处理的移植肢体在血小板积聚方面观察到的差异值得在人类中进一步研究该技术。
