抗鸡淋巴细胞表面抗原单克隆抗体的制备与鉴定

Production and characterization of monoclonal antibodies against chicken lymphocyte surface antigens.

作者信息

Kondo T, Hattori M, Kodama H, Onuma M, Mikami T

机构信息

Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.

出版信息

Nihon Juigaku Zasshi. 1990 Feb;52(1):97-103. doi: 10.1292/jvms1939.52.97.

DOI:10.1292/jvms1939.52.97

PMID:2138236

Abstract

A panel of monoclonal antibodies (mAbs) with specificity for chicken lymphocyte surface antigens was established and characterized based on their reactivities against chicken lymphoid cells and tumor cell lines on flow cytometry. Three mAbs (7-3G-2, 7-2E-8, and JB-2) reacted preferentially with thymocytes, however, none of them reacted with Marek's disease derived T lymphoblastoid cell lines. Four mAbs (6-27A-1, 4-5C-5, Lc-4, and Lc-6) reacted with spleen cells and peripheral blood leukocytes as well as thymocytes. All seven mAbs reacted with chicken embryonic thymocytes from day 12 of embryonic life onward. All mAbs showed no reactivity against bursal lymphocytes.

摘要

建立了一组对鸡淋巴细胞表面抗原具有特异性的单克隆抗体（mAb），并根据它们在流式细胞仪上对鸡淋巴样细胞和肿瘤细胞系的反应性对其进行了表征。三种单克隆抗体（7-3G-2、7-2E-8和JB-2）优先与胸腺细胞反应，然而，它们均未与马立克氏病衍生的T淋巴母细胞系反应。四种单克隆抗体（6-27A-1、4-5C-5、Lc-4和Lc-6）与脾细胞、外周血白细胞以及胸腺细胞反应。所有七种单克隆抗体从胚胎期第12天起就与鸡胚胎胸腺细胞反应。所有单克隆抗体对法氏囊淋巴细胞均无反应。