CEIT, Paseo de Manuel Lardizábal, N° 15, 20018 Donostia-San Sebastián, Spain.
Biosens Bioelectron. 2011 Apr 15;26(8):3705-9. doi: 10.1016/j.bios.2011.02.013. Epub 2011 Feb 16.
An immunomagnetic method for the selective and quantitative detection of biological species by means of a magnetoresistive biosensor and superparamagnetic particles has been optimized. In order to achieve this, a giant magnetoresistive Co (5.10nm)/Cu (2.47 nm) multilayer structure has been chosen as the sensitive material, showing a magnetoresistance of 3.60% at 215 Oe and a sensitivity up to 0.19 Ω/Oe between 145 Oe and 350 Oe. The outward gold surface of the sensor is biofunctionalized with a Self-Assembled Monolayer (SAM). In addition, three different types of magnetic labels have been tested. 2 μm diameter magnetic carriers (7.68 pg ferrite/particle) have shown the best response and they have induced a shift in the magnetoresistive hysteresis loops up to 9% at 175 Oe.
已经优化了一种免疫磁方法，通过磁电阻生物传感器和超顺磁粒子来选择性和定量检测生物物种。为此，选择了巨磁电阻Co(5.10nm)/Cu(2.47nm)多层结构作为敏感材料，在 215 奥斯特时具有 3.60%的磁电阻，在 145 奥斯特至 350 奥斯特之间的灵敏度高达 0.19 欧姆/奥斯特。传感器的外向金表面通过自组装单分子层(SAM)进行生物功能化。此外，还测试了三种不同类型的磁性标签。直径为 2μm 的磁性载体(每个颗粒 7.68pg 铁氧体)显示出最佳响应，它们在 175 奥斯特时将磁电阻滞后环的偏移诱导至 9%。
