Department of Anesthesiology and Critical Care Medicine, Division of Pulmonary and Critical Care Medicine in the Department of Medicine, and Division of Physiology in the Department of Environmental Health Sciences, The Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA.
J Breath Res. 2009 Mar;3(1):016002. doi: 10.1088/1752-7155/3/1/016002. Epub 2009 Mar 10.
Reactive oxygen species are associated with tissue inflammation and injury. Our laboratory has demonstrated that ethane, a stable product of lipid peroxidation, in exhaled breath can be used to measure total body oxidative stress. Herein patients were studied who underwent outpatient surgery, laproscopic bilateral tubal ligation (BTL, n = 10) and anterior cruciate ligament (ACL, n = 10) repair of the knee. These surgical procedures were expected to involve mild degrees of ischemia and reperfusion. In each of these cases propofol, an intravenous anesthetic with antioxidant properties, was used. Breath ethane was measured as a biomarker of oxidative stress that occurred at reperfusion of ischemic tissue. Data were analyzed by one-way analysis of variance. Clinically relevant concentrations of propofol were unable to completely block the increase in oxidative stress following reperfusion in either of these minor surgeries. Breath ethane increased significantly after reperfusion in both the BTL (p = 0.03) and the ACL (p = 0.005) patients. Also, the increase in oxidative stress was related to the time of ischemia.
活性氧与组织炎症和损伤有关。我们的实验室已经证明，呼气中稳定的脂质过氧化产物乙烷可用于测量全身氧化应激。在此，我们研究了接受门诊手术的患者，腹腔镜双侧输卵管结扎术（BTL，n=10）和膝关节前交叉韧带（ACL）修复术（n=10）。这些手术预计会涉及到轻度的缺血再灌注。在这些病例中，均使用了具有抗氧化特性的静脉麻醉药异丙酚。乙烷呼气被用作氧化应激的生物标志物，该标志物发生在缺血组织再灌注时。通过单向方差分析对数据进行分析。在这两种较小的手术中，临床相关浓度的异丙酚均无法完全阻止再灌注后氧化应激的增加。BTL（p=0.03）和 ACL（p=0.005）患者在再灌注后乙烷呼气明显增加。此外，氧化应激的增加与缺血时间有关。
