Department of Anesthesiology and Critical Care Medicine, Division of Pulmonary and Critical Care Medicine in the Department of Medicine, and Division of Physiology in the Department of Environmental Health Sciences, The Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA.
J Breath Res. 2009 Mar;3(1):016003. doi: 10.1088/1752-7155/3/1/016003. Epub 2009 Mar 10.
Reactive oxygen species are associated with tissue inflammation and injury. Our laboratory has demonstrated that ethane, a stable product of lipid peroxidation, in exhaled breath can be used to measure total body oxidative stress. An ischemia-reperfusion model of lung injury in sheep has been studied in which pulmonary and bronchial lung perfusion could be interrupted and restored. The goal of this study was to investigate whether two commercial formulations of propofol and the individual components of the commercial formulations attenuated the oxidative stress produced in this model. Breath ethane and breath carbon monoxide were measured as biomarkers of oxidative stress that occur at reperfusion of ischemic tissue. Data were analyzed by a standard least-squares-fit model. One of the formulations for propofol, which contained the preservative ethylenediaminetetraacetic acid (EDTA), was found to decrease the overall level of oxidative stress in sheep. Furthermore, while several models of severe lung injury demonstrate additional production of reactive oxygen species, our model of ischemia/reperfusion of lung tissue did not.
活性氧与组织炎症和损伤有关。我们的实验室已经证明，呼气中的乙烷（脂质过氧化的稳定产物）可用于测量全身氧化应激。本研究中研究了一种绵羊肺损伤的缺血再灌注模型，在此模型中可以中断和恢复肺和支气管肺灌注。本研究的目的是研究两种商业配方的丙泊酚及其商业配方的各个成分是否能减轻该模型产生的氧化应激。呼气中的乙烷和呼气中的一氧化碳被作为氧化应激的生物标志物来测量，这些标志物发生在缺血组织的再灌注期间。数据通过标准最小二乘拟合模型进行分析。一种含有防腐剂乙二胺四乙酸（EDTA）的丙泊酚配方被发现可降低绵羊的整体氧化应激水平。此外，虽然几种严重肺损伤模型显示出更多的活性氧生成，但我们的肺组织缺血/再灌注模型没有。
