Central Laboratory, 3-1-1 Numakage, Minami-Ku, Saitama 336-8601, Japan.
J Breath Res. 2010 Jun;4(2):026005. doi: 10.1088/1752-7155/4/2/026005. Epub 2010 Apr 9.
Recently, we reported that myrsinoic acid B purified from Myrsine seguinii inhibited methyl mercaptan (CH(3)SH) production by Fusobacterium nucleatum JCM8532. Since hydrogen sulfide (H(2)S) is the main component of physiological halitosis, while CH(3)SH is involved in pathological oral halitosis, the objective of this study is to determine whether myrsinoic acid B inhibits H(2)S production by oral microorganisms. F. nucleatum, Porphyromonas gingivalis and Treponema denticola were incubated with myrsinoic acid B and a substrate such as l-cysteine or l-methionine. H(2)S or CH(3)SH concentration in the headspace air, was determined using a gas chromatograph. The concentration of myrsinoic acid B inhibiting 50% (IC(50)) of H(2)S production by F. nucleatum was 0.142 µg ml(-1), and the IC(50) of P. gingivalis and T. denticola were 2.71 µg ml(-1) and 28.9 µg ml(-1), respectively. The presence of pyruvate, a by-product of H(2)S production, was determined. The IC(50) values of myrsinoic acid B for pyruvate production were 22.9 µg ml(-1) for F. nucleatum, 87.7 µg ml(-1) for P. gingivalis and 165 µg ml(-1) for T. denticola. We concluded that myrsinoic acid B inhibited the production of both H(2)S and pyruvate by periodontal pathogens.
最近，我们报道了从紫金牛属植物中分离得到的杨梅酸 B 可以抑制产甲烷短杆菌 JCM8532 产生甲基硫醇（CH(3)SH）。由于硫化氢（H(2)S）是生理性口臭的主要成分，而 CH(3)SH 则与病理性口臭有关，因此本研究旨在确定杨梅酸 B 是否抑制口腔微生物产生 H(2)S。将产甲烷短杆菌、牙龈卟啉单胞菌和牙髓密螺旋体与杨梅酸 B 和半胱氨酸或蛋氨酸等底物孵育。使用气相色谱仪测定顶空气体中 H(2)S 或 CH(3)SH 的浓度。杨梅酸 B 抑制产甲烷短杆菌产生 H(2)S 的 50%（IC(50)）浓度为 0.142 µg ml(-1)，而 P. gingivalis 和 T. denticola 的 IC(50)分别为 2.71 µg ml(-1)和 28.9 µg ml(-1)。还测定了 H(2)S 生成的副产物丙酮酸的存在。杨梅酸 B 对产甲烷短杆菌丙酮酸生成的 IC(50)值为 22.9 µg ml(-1)，对牙龈卟啉单胞菌为 87.7 µg ml(-1)，对牙髓密螺旋体为 165 µg ml(-1)。我们得出结论，杨梅酸 B 抑制牙周病原体产生 H(2)S 和丙酮酸。
