Tang-Larsen J, Claesson R, Edlund M B, Carlsson J
Department of Oral Microbiology, University of Umeå, Sweden.
J Periodontal Res. 1995 Nov;30(6):390-5. doi: 10.1111/j.1600-0765.1995.tb01292.x.
We recently studied the utilization of glutathione (L-gamma-glutamyl-L-cysteinylglycine), L-cysteinylglycine and L-cysteine by anaerobic bacteria. The rate of hydrogen sulfide formation from these compounds was determined and it was concluded that Peptostreptococcus micros and Fusobacterium nucleatum subsp. nucleatum had an active transport of small peptides. In the present study it is shown that methyl mercaptan formation from L-methionine and L-methionyl-containing peptides can also be used to study peptide utilization. There were differences among the periodontal bacteria P. micros, F. nucleatum subsp. nucleatum, and Porphyromonas gingivalis in their capacity to use L-cysteine and L-methionine and peptides containing these amino acids. The peptides were used more efficiently by P. micros and F. nucleatum subsp. nucleatum than by P. gingivalis. All three species used the peptides more efficiently than the free amino acids. The efficiency in utilizing various amino acids and peptides may be among the key determinants of the periodontal microbial ecology.
我们最近研究了厌氧菌对谷胱甘肽（L-γ-谷氨酰-L-半胱氨酰甘氨酸）、L-半胱氨酰甘氨酸和L-半胱氨酸的利用情况。测定了这些化合物生成硫化氢的速率，并得出结论：微小消化链球菌和具核梭杆菌具核亚种具有小肽的主动转运功能。在本研究中表明，由L-蛋氨酸和含L-蛋氨酰的肽生成甲硫醇也可用于研究肽的利用情况。在利用L-半胱氨酸、L-蛋氨酸以及含有这些氨基酸的肽的能力方面，牙周细菌微小消化链球菌、具核梭杆菌具核亚种和牙龈卟啉单胞菌之间存在差异。微小消化链球菌和具核梭杆菌具核亚种利用肽的效率高于牙龈卟啉单胞菌。所有这三个菌种利用肽的效率都高于游离氨基酸。利用各种氨基酸和肽的效率可能是牙周微生物生态的关键决定因素之一。
