Stowers Institute for Medical Research, Kansas City, Missouri, United States of America.
PLoS Genet. 2011 Feb;7(2):e1002005. doi: 10.1371/journal.pgen.1002005. Epub 2011 Feb 24.
Homologous recombination in meiosis is initiated by the programmed induction of double strand breaks (DSBs). Although the Drosophila Spo11 ortholog Mei-W68 is required for the induction of DSBs during meiotic prophase, only one other protein (Mei-P22) has been shown to be required for Mei-W68 to exert this function. We show here that the chromatin-associated protein Trade Embargo (Trem), a C2H2 zinc finger protein, is required to localize Mei-P22 to discrete foci on meiotic chromosomes, and thus to promote the formation of DSBs, making Trem the earliest known function in the process of DSB formation in Drosophila oocytes. We speculate that Trem may act by either directing the binding of Mei-P22 to preferred sites of DSB formation or by altering chromatin structure in a manner that allows Mei-P22 to form foci.
减数分裂中的同源重组是由双链断裂（DSBs）的程序性诱导引发的。尽管果蝇 Spo11 同源物 Mei-W68 是在减数分裂前期诱导 DSBs 所必需的，但只有另一种蛋白质（Mei-P22）被证明是 Mei-W68 发挥此功能所必需的。我们在这里表明，染色质相关蛋白 Trade Embargo（Trem），一种 C2H2 锌指蛋白，需要将 Mei-P22 定位到减数分裂染色体上的离散焦点上，从而促进 DSB 的形成，使 Trem 成为在果蝇卵母细胞中 DSB 形成过程中最早已知的功能。我们推测 Trem 可能通过以下两种方式之一发挥作用：指导 Mei-P22 与 DSB 形成的首选位点结合，或通过改变染色质结构，使 Mei-P22 能够形成焦点。
