Stowers Institute for Medical Research, Kansas City, Missouri, United States of America.
Waksman Institute and Department of Genetics, Rutgers, the State University of New Jersey, Piscataway, New Jersey, United States of America.
PLoS Genet. 2019 Jan 7;15(1):e1007886. doi: 10.1371/journal.pgen.1007886. eCollection 2019 Jan.
Meiotic recombination, which is necessary to ensure that homologous chromosomes segregate properly, begins with the induction of meiotic DNA double-strand breaks (DSBs) and ends with the repair of a subset of those breaks into crossovers. Here we investigate the roles of two paralogous genes, CG12200 and CG31053, which we have named Narya and Nenya, respectively, due to their relationship with a structurally similar protein named Vilya. We find that narya recently evolved from nenya by a gene duplication event, and we show that these two RING finger domain-containing proteins are functionally redundant with respect to a critical role in DSB formation. Narya colocalizes with Vilya foci, which are known to define recombination nodules, or sites of crossover formation. A separation-of-function allele of narya retains the capacity for DSB formation but cannot mature those DSBs into crossovers. We further provide data on the physical interaction of Narya, Nenya and Vilya, as assayed by the yeast two-hybrid system. Together these data support the view that all three RING finger domain-containing proteins function in the formation of meiotic DNA DSBs and in the process of crossing over.
减数分裂重组对于确保同源染色体正确分离是必需的，它始于诱导减数分裂 DNA 双链断裂 (DSB)，并以修复这些断裂中的一部分形成交叉结束。在这里，我们研究了两个平行基因 CG12200 和 CG31053 的作用，我们分别将它们命名为 Narya 和 Nenya，这是由于它们与一种结构相似的蛋白质 Vilya 有关。我们发现，narya 是由基因复制事件从 nenya 中最近进化而来的，我们表明这两种含 RING 指结构域的蛋白质在 DSB 形成的关键作用上是功能冗余的。Narya 与 Vilya 焦点共定位，Vilya 焦点已知定义了重组结或交叉形成的位点。narya 的功能分离等位基因保留了 DSB 形成的能力，但不能将这些 DSB 成熟为交叉。我们进一步提供了通过酵母双杂交系统测定的 Narya、Nenya 和 Vilya 之间的物理相互作用的数据。这些数据共同支持了所有三种含 RING 指结构域的蛋白质都参与了减数分裂 DNA DSB 的形成和交叉过程的观点。
