一种可以合成硫代吡嗪的真菌非核糖体肽合成酶模块。
A fungal nonribosomal peptide synthetase module that can synthesize thiopyrazines.
机构信息
Department of Chemical and Biomolecular Engineering, University of California Los Angeles, Los Angeles, California 90095, United States.
出版信息
Org Lett. 2011 Apr 1;13(7):1758-61. doi: 10.1021/ol200288w. Epub 2011 Mar 8.
Abstract
A nonribosomal peptide synthetase-like enzyme (NRPS325) from Aspergillus terreus was reconstituted in vitro and was shown to synthesize thiopyrazines using an unprecedented mechanism. Substrate promiscuity of NRPS325 toward different amino acids and free thiols was explored to produce >60 different thiopyrazine compounds.
摘要
土曲霉中的一种非核糖体肽合成酶样酶（NRPS325）在体外重新构建，并显示出使用前所未有的机制合成噻嗪的能力。探索了 NRPS325 对不同氨基酸和游离巯基的底物混杂性，以产生超过 60 种不同的噻嗪化合物。
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