Department of Physiotherapy, Superior School of Cruzeiro, Cruzeiro, São Paulo, Brazil.
J Oral Pathol Med. 2011 Jul;40(6):467-75. doi: 10.1111/j.1600-0714.2011.01023.x. Epub 2011 Mar 8.
The enzyme fatty acid synthase plays a fundamental role in the biosynthesis of fatty acids. Several recent studies have demonstrated a high fatty acid synthase expression in malignant tumors. Few studies have been conducted in oral and salivary gland tumors describing the fatty acid synthase expression.
This study evaluated and compared, by immunohistochemical reaction, the expression of fatty acid synthase and Ki-67 in salivary gland tumors. The immunohistochemical study used the streptavidin-biotin-peroxidase technique, with antibodies anti-fatty acid synthase and anti-Ki-67. The fatty acid synthase was analyzed by scores, considering the intensity of labeling, quantity of labeled cells and histological component. The Ki-67 was analyzed by counting of one thousand cells, calculating the quantity of positive cells in regions with higher density of labeling. Statistical analysis was performed using the Pearson and Mann-Whitney correlation tests.
There was greater fatty acid synthase expression in pleomorphic adenoma compared to other tumors, and predominance of Ki-67 in malignant tumors. Among these, the mucoepidermoid carcinoma presented the highest proliferation rate. The expression of fatty acid synthase and Ki-67 did not present correlation between the tumors analyzed, except in pleomorphic adenomas, with statistically significant relationship between them.
It is suggested that the fat metabolism in salivary gland tumors is related to maintenance of cell differentiation. Its expression prevailed in benign tumors, while Ki-67 prevailed in the mucoepidermoid carcinoma, demonstrating its high proliferation rate, followed by the cystic adenoid carcinoma and polymorphous low grade adenocarcinoma.
脂肪酸合酶在脂肪酸的生物合成中起着基本作用。最近的几项研究表明，脂肪酸合酶在恶性肿瘤中表达较高。关于口腔和唾液腺肿瘤中脂肪酸合酶的表达，研究较少。
本研究通过免疫组织化学反应评估并比较了唾液腺肿瘤中脂肪酸合酶和 Ki-67 的表达。免疫组织化学研究使用链霉亲和素-生物素-过氧化物酶技术，使用抗脂肪酸合酶和抗 Ki-67 抗体。脂肪酸合酶通过评分进行分析，考虑标记的强度、标记细胞的数量和组织学成分。Ki-67 通过对一千个细胞进行计数来分析，计算标记密度较高区域中阳性细胞的数量。使用 Pearson 和 Mann-Whitney 相关检验进行统计分析。
多形性腺瘤中脂肪酸合酶的表达高于其他肿瘤，恶性肿瘤中 Ki-67 的表达较高。其中，黏液表皮样癌的增殖率最高。除多形性腺瘤外，分析的肿瘤中脂肪酸合酶和 Ki-67 的表达之间没有相关性，它们之间存在统计学意义上的关系。
提示唾液腺肿瘤中的脂肪代谢与细胞分化的维持有关。其表达在良性肿瘤中占优势，而 Ki-67 在黏液表皮样癌中占优势，表明其增殖率较高，其次是囊性腺样癌和多形性低度恶性腺癌。
