AstraZeneca Research & Development, Early Clinical Development, Mölndal, Sweden.
Dis Esophagus. 2011 Sep;24(7):462-9. doi: 10.1111/j.1442-2050.2011.01181.x. Epub 2011 Mar 8.
Bioimpedance spectroscopy can identify pathological changes related to precancerous lesions of the cervix uteri and esophagus. It therefore has the potential to detect early reflux-related changes in the esophageal mucosa, such as dilated intercellular spaces. The reliable detection of dilated intercellular spaces at the time of endoscopy would yield a significant diagnostic advantage for separating patients with functional heartburn from the large proportion of patients with gastroesophageal reflux symptoms but no macroscopic esophagitis or pathological acid exposure. The bioimpedance of the esophageal mucosa, measured with a small caliber probe, was evaluated in a series of preclinical experiments. First, sections of rabbit esophageal epithelium were mounted in Ussing chambers and exposed to solutions at pH 7.4 or pH 1.5 for 45 minutes. Impedance measurements were taken at varying probe pressures. Second, rabbit esophageal epithelia were perfused for 45 minutes in situ with pH 1.1 or control solutions and impedance measurements taken. Samples from both in vitro and in situ experiments were taken for morphological examination by light microscopy. Finally, esophageal bioimpedance was measured in awake dogs with permanent esophagocutaneous stoma. The in situ experiments demonstrated that morphological changes in the esophageal mucosa could be discerned by the use of bioimpedance spectroscopy. The variability in resistivity was species-independent but was affected by the pressure applied to the probe. The results suggest that evaluation of bioimpedance spectroscopy for use in a clinical setting is warranted. Small morphological differences in the esophageal mucosa may be detected by the use of bioimpedance spectroscopy.
生物阻抗光谱学可以识别与宫颈癌和食管癌癌前病变相关的病理性变化。因此，它有可能检测到食管黏膜早期与反流相关的变化，如细胞间隙扩张。在进行内窥镜检查时可靠地检测到细胞间隙扩张，将为区分功能性烧心患者和大多数具有胃食管反流症状但无宏观食管炎或病理性酸暴露的患者提供重要的诊断优势。使用小口径探头测量食管黏膜的生物阻抗，在一系列临床前实验中进行了评估。首先，将兔食管上皮组织切片置于 Ussing 室中，并在 pH 值为 7.4 或 1.5 的溶液中暴露 45 分钟。在不同的探头压力下进行阻抗测量。其次，将兔食管上皮在 pH 值为 1.1 或对照溶液中在位灌注 45 分钟，并进行阻抗测量。从体外和体内实验中取样，通过光镜进行形态学检查。最后，在具有永久性食管皮瘘的清醒狗中测量食管生物阻抗。体内实验表明，生物阻抗光谱学可用于识别食管黏膜的形态变化。电阻率的可变性与物种无关，但受探头施加的压力影响。结果表明，有必要评估生物阻抗光谱学在临床环境中的应用。生物阻抗光谱学可能检测到食管黏膜的微小形态差异。
