Quality and Safety Department, Nestlé Research Centre, Lausanne, Switzerland.
Anal Biochem. 2011 Jul 1;414(1):166-8. doi: 10.1016/j.ab.2011.03.002. Epub 2011 Mar 6.
Analysis of 2-aminobenzamide-labeled oligosaccharides requires removal of excess labeling reagents before chromatography. Manual cleanup is time-consuming and not optimal for routine analysis, so an on-line solid-phase extraction was developed. Labeled oligosaccharides are trapped on an amide phase in a small guard column, the excess reagents are washed away, and then the sample is transferred to the analytical column for analysis. The on-line protocol shortened the sample preparation time and has been applied for the analysis of oligosaccharides and N-glycans released from glycoproteins.
分析 2-氨基苯甲酰胺标记的寡糖需要在色谱分析前除去多余的标记试剂。手动清洗既费时又不适合常规分析，因此开发了一种在线固相萃取法。标记的寡糖被捕获在一个小保护柱的酰胺相中，多余的试剂被冲洗掉，然后将样品转移到分析柱中进行分析。在线方案缩短了样品制备时间，并已应用于从糖蛋白中释放的寡糖和 N-糖链的分析。
