Aebischer T, Oehen S, Hengartner H
Department of Experimental Pathology, University Hospital, Zürich, Switzerland.
Eur J Immunol. 1990 Mar;20(3):523-31. doi: 10.1002/eji.1830200310.
Correlations between the T cell receptor (TcR) V gene usage and the specificity of T cells have been primarily described for major histocompatibility complex (MHC) class II-restricted helper T cell responses. In the present study the TcR genes expressed by MHC class I-restricted murine cytotoxic T cells (CTL) specific for a major epitope of the lymphocytic choriomeningitis virus (LCMV), LCMV-GP2(275-289), were investigated. The TcR primary structure of an LCMV-GP2(275-289) specific H-2Db-restricted CTL clone has been determined. It uses a member of the V alpha 4 family joined to J alpha AN14.4 for the alpha chain and V beta 10 rearranged to D beta 2.1 and J beta 2.4 for its beta chain. Four other independent LCMV-GP2(275-289) specific H-2Db-restricted CTL clones also expressed V alpha 4 and V beta 10 gene elements. Furthermore, V alpha 4 and V beta 10 were preferentially expressed by polyclonal CTL of C57BL/6 origin specific for LCMV. These results suggest that both TcR V alpha and V beta regions are important for the recognition of the LCMV-GP2(275-289) epitope on H-2Db molecules.
T细胞受体（TcR）V基因使用情况与T细胞特异性之间的相关性，主要是针对主要组织相容性复合体（MHC）II类限制性辅助性T细胞反应进行描述的。在本研究中，对针对淋巴细胞性脉络丛脑膜炎病毒（LCMV）主要表位LCMV-GP2(275 - 289)的MHC I类限制性小鼠细胞毒性T细胞（CTL）所表达的TcR基因进行了研究。已确定了一个LCMV-GP2(275 - 289)特异性H-2Db限制性CTL克隆的TcR一级结构。其α链使用Vα4家族的一个成员与JαAN14.4连接，β链则是Vβ10重排至Dβ2.1和Jβ2.4。另外四个独立的LCMV-GP2(275 - 289)特异性H-2Db限制性CTL克隆也表达Vα4和Vβ10基因元件。此外，针对LCMV的C57BL/6来源的多克隆CTL优先表达Vα4和Vβ10。这些结果表明，TcR的Vα和Vβ区域对于识别H-2Db分子上的LCMV-GP2(275 - 289)表位都很重要。
