Zola H, Melo J V, Zowtyj H N, Nikoloutsopoulos A, Skinner J
Department of Clinical Immunology, Flinders Medical Centre, Bedford Park, South Australia.
Hum Immunol. 1990 Apr;27(4):368-77. doi: 10.1016/0198-8859(90)90087-6.
In view of the changes reported in expression of the different members of the leukocyte-common family of antigens by T cells as they are activated, B cells were examined with monoclonal antibodies against p220-180 (CD45), p220,205 (CD45R), and p180 (UCHL1). Resting tonsil B cells reacted with CD45 and CD45R antibodies, but not with UCHL1. Activation of B cells by anti-IgM and IL-4 and culture of activated B cells with factors which induce proliferation (B-cell growth factor) and differentiation (IL-6) led to only minor changes in the expression of CD45, CD45R, and p180 antigens. Activation with phorbol ester led to minor increases in reactivity with CD45 and CD45R antibodies and led to a weak reactivity with UCHL1. The results suggest that the functional relationship previously described in T cells between p180 and p220,205 is not seen in B cells. p180 is induced, but only at low levels and only with nonphysiologic activation. CD45R is not lost under the same conditions. However, in terminally differentiated B cells, and in some chronic lymphocytic leukemias, p180 expression was seen, often coexpressed with CD45R.
鉴于T细胞被激活时白细胞共同抗原家族不同成员的表达变化，我们用抗p220 - 180（CD45）、p220,205（CD45R）和p180（UCHL1）的单克隆抗体检测了B细胞。静息的扁桃体B细胞与CD45和CD45R抗体发生反应，但不与UCHL1反应。用抗IgM和IL - 4激活B细胞，并将激活的B细胞与诱导增殖（B细胞生长因子）和分化（IL - 6）的因子一起培养，导致CD45、CD45R和p180抗原的表达仅发生轻微变化。用佛波酯激活导致与CD45和CD45R抗体的反应性略有增加，并导致与UCHL1的弱反应性。结果表明，先前在T细胞中描述的p180与p220,205之间的功能关系在B细胞中未观察到。p180被诱导，但仅在低水平且仅在非生理性激活时被诱导。在相同条件下，CD45R不会丢失。然而，在终末分化的B细胞以及一些慢性淋巴细胞白血病中，可见p180表达，且常与CD45R共表达。
