Akbar A N, Terry L, Timms A, Beverley P C, Janossy G
Department of Immunology, Royal Free Hospital School of Medicine, London, United Kingdom.
J Immunol. 1988 Apr 1;140(7):2171-8.
A group of mAb recognizing the 200- and/or 220-kDa determinants (CD45R) of the leukocyte common Ag such as 2H4, WR16, MD4.3, and SN130 cross-block each other showing that they recognize a closely related epitope. The antibody UCHL1 reacts with a 180-kDa determinant of the leukocyte common Ag and exhibits a reciprocal T subset distribution pattern to the CD45R group. Peripheral blood T cells were 40% positive for UCHL1 and 58 to 65% positive for the CD45R antibodies; less than 1% of cells stained for both. On activation of CD45R+,UCHL1- T cells by PHA, up to 40% of cells became positive for both CD45R and UCHL1 by day 3. By day 7, CD45R+,UCHL1- cells fell from 90 to less than 21% whereas UCHL1+,CD45R- cells rose from 2 to 93%. Conversely, PHA-stimulated UCHL1+,CD45R- cells remained UCHL1+,CD45R- during the 7 days in culture showing that phenotypic change was unidirectional from CD45R+ to UCHL1+. In primary allogeneic mixed lymphocyte reactions, activated CD45R+ T cells also showed a change to UCHL1+. When these cells were rechallenged by the original alloantigen, the UCHL1+ cells showed 7- to 20-fold greater proliferation than the CD45R+ cells on day 3 after rechallenge. The recovery of virtually all alloantigen induced secondary proliferative response in the UCHL1+,CD45R- T cell population suggests that UCHL1 identifies a primed population of T cells which may include memory cells.
一组识别白细胞共同抗原200 kDa和/或220 kDa决定簇（CD45R）的单克隆抗体，如2H4、WR16、MD4.3和SN130，彼此间存在交叉阻断，表明它们识别的是密切相关的表位。抗体UCHL1与白细胞共同抗原的180 kDa决定簇发生反应，并且与CD45R组呈现相反的T细胞亚群分布模式。外周血T细胞中，UCHL1阳性率为40%，CD45R抗体阳性率为58%至65%；同时被两种抗体染色的细胞不到1%。用PHA激活CD45R⁺、UCHL1⁻ T细胞后，到第3天，高达40%的细胞同时变为CD45R和UCHL1阳性。到第7天，CD45R⁺、UCHL1⁻细胞从90%降至不到21%，而UCHL1⁺、CD45R⁻细胞从2%升至93%。相反，PHA刺激的UCHL1⁺、CD45R⁻细胞在培养的7天内仍保持UCHL1⁺、CD45R⁻，表明表型变化是从CD45R⁺到UCHL1⁺的单向变化。在原发性同种异体混合淋巴细胞反应中，活化的CD45R⁺ T细胞也显示出向UCHL1⁺的转变。当这些细胞再次受到原始同种异体抗原刺激时，再次刺激后第3天，UCHL1⁺细胞的增殖比CD45R⁺细胞高7至20倍。UCHL1⁺、CD45R⁻ T细胞群体中几乎所有同种异体抗原诱导的二次增殖反应的恢复表明，UCHL1识别的是一群已致敏的T细胞，其中可能包括记忆细胞。
