噬菌体T7 RNA聚合酶的单晶。

Single crystals of bacteriophage T7 RNA polymerase.

作者信息

Sousa R, Rose J P, Chung Y J, Lafer E M, Wang B C

机构信息

Department of Crystallography, University of Pittsburgh, Pennsylvania 15260.

出版信息

Proteins. 1989;5(4):266-70. doi: 10.1002/prot.340050403.

DOI:10.1002/prot.340050403

PMID:2678097

Abstract

Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 x 0.3 x 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which is enzymatically active upon dissolution. These crystals belong to the monoclinic space group P2(1) and have unit cell parameters a = 114.5 A, b = 139.6 A, c = 125.7 A, and beta = 98.1 degrees. Self-rotation function studies indicate that there are three molecules per asymmetric unit. The crystals diffract to at least 3.0 A resolution. These are the first crystals of a DNA-dependent RNA polymerase suitable for high-resolution X-ray structure determination.

摘要

T7 RNA聚合酶的单晶已生长到最大尺寸为1.8×0.3×0.3毫米。这些晶体由完全完整的T7 RNA聚合酶组成，溶解后具有酶活性。这些晶体属于单斜空间群P2(1)，其晶胞参数为a = 114.5埃，b = 139.6埃，c = 125.7埃，β = 98.1度。自旋转函数研究表明，每个不对称单元中有三个分子。这些晶体的衍射分辨率至少为3.0埃。这是适合高分辨率X射线结构测定的DNA依赖性RNA聚合酶的首批晶体。