Institute of Molecular Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany.
Arch Virol. 2011 Jun;156(6):1059-63. doi: 10.1007/s00705-011-0953-8. Epub 2011 Mar 9.
Koi herpesvirus (KHV) causes a fatal disease in koi and common carp, but no reliable and genetically characterized vaccines are available up to now. Therefore, we generated KHV recombinants possessing deletions within the viral ribonucleotide reductase (RNR), thymidine kinase (TK), dUTPase, or TK and dUTPase genes, and their corresponding rescuants. All KHV mutants were replication competent in cultured cells. Whereas plaque sizes and titers of RNR-negative KHV were reduced, replication of the other mutants was not affected. Experimental infection of carp indicated attenuation of TK- or dUTPase-deleted KHV, and PCR analysis of tissue samples permitted differentiation of mutant from wild-type virus.
锦鲤疱疹病毒（KHV）可引起锦鲤和鲤鱼的致命疾病，但迄今为止尚无可靠的、具有遗传特征的疫苗。因此，我们生成了具有病毒核糖核苷酸还原酶（RNR）、胸苷激酶（TK）、dUTP 酶或 TK 和 dUTP 酶基因缺失的 KHV 重组体及其相应的拯救体。所有 KHV 突变体在培养细胞中均具有复制能力。尽管 RNR 阴性 KHV 的蚀斑大小和滴度降低，但其他突变体的复制不受影响。鲤鱼的实验感染表明 TK 或 dUTP 酶缺失的 KHV 减毒，并且组织样本的 PCR 分析允许突变体与野生型病毒区分开来。
