Shukla Kamla Kant, Mahdi Abbas Ali, Mishra Vivek, Rajender Singh, Sankhwar Satya Narain, Patel Devender, Das Mukul
Department of Biochemistry, C.S.M. Medical University, Lucknow 226003, UP, India.
Food Toxicology Division, Indian Institute of Toxicology Research, Lucknow, UP, India.
Reprod Biomed Online. 2011 May;22(5):421-7. doi: 10.1016/j.rbmo.2011.01.010. Epub 2011 Mar 8.
This study investigated the effect of a 3-month treatment with Withania somnifera on apoptosis and intracellular reactive oxygen species (ROS) concentration of spermatozoa and the metal ions copper, zinc, iron and gold in seminal plasma from infertile men (normozoospermic, n=25; oligozoospermic, n=25; and asthenozoospermic, n=25). The apoptotic and necrotic cell distribution were analysed by annexin-V binding and propidium iodide uptake using flow cytometry. ROS generation was measured by fluorescence intensity and metal ions were analysed by atomic absorption spectrophotometry. The results demonstrated that, prior to treatment, sperm apoptosis and intracellular ROS concentrations were significantly higher in all groups of infertile men compared with controls (P<0.01 to P<0.001). Similarly, the concentrations of the essential metal ions Cu(2+), Zn(2+), Fe(2+) and Au(2+) in seminal plasma were lower. Treatment with W. somnifera significantly reduced apoptosis in normozoospermic and oligozoospermic men and ROS concentrations in oligozoospermic and asthenozoospermic men (all P<0.05). Treatment also significantly improved metal ion concentrations in infertile men (P<0.01). It is concluded that W. somnifera improves semen quality by reducing oxidative stress and cell death, as well as improving essential metal ion concentrations. The aim of this study was to investigate the effect of 3-month treatment with Withania somnifera on apoptosis and intracellular reactive oxygen species (ROS) concentration in spermatozoa from infertile men. Before and following treatment, sperm apoptosis and concentrations of intracellular ROS and the metal ions copper, zinc, iron, and gold in seminal plasma were measured. The apoptotic and necrotic cell distribution were analysed by annexin-V binding and propidium iodide uptake using flow cytometry. ROS generation was measured by fluorescence intensity and metal ions were analysed by atomic absorption spectrophotometry. The results demonstrated that prior, to treatment, apoptosis and intracellular ROS concentrations were significantly higher in all groups of infertile men compared with controls. Similarly, the concentrations of the essential metal ions Cu(2+), Zn(2+), Fe(2+) and Au(2+) in seminal plasma were lower. Treatment with W. somnifera significantly reduced apoptosis and ROS concentrations and improved metal ion concentrations in infertile subjects. It is concluded that W. somnifera improves semen quality by reducing oxidative stress and cell death and improving essential metal ion concentrations.
本研究调查了用印度人参进行为期3个月的治疗对不育男性（正常精子症患者，n = 25；少精子症患者，n = 25；弱精子症患者，n = 25）精液中精子的凋亡、细胞内活性氧（ROS）浓度以及精浆中金属离子铜、锌、铁和金的影响。通过使用流式细胞术检测膜联蛋白-V结合和碘化丙啶摄取来分析凋亡和坏死细胞分布。通过荧光强度测量ROS生成，并通过原子吸收分光光度法分析金属离子。结果表明，在治疗前，与对照组相比，所有不育男性组的精子凋亡和细胞内ROS浓度均显著更高（P<0.01至P<0.001）。同样，精浆中必需金属离子Cu(2+)、Zn(2+)、Fe(2+)和Au(2+)的浓度较低。用印度人参治疗显著降低了正常精子症和少精子症男性的凋亡以及少精子症和弱精子症男性的ROS浓度（均P<0.05）。治疗还显著提高了不育男性的金属离子浓度（P<0.01）。得出的结论是，印度人参通过降低氧化应激和细胞死亡以及提高必需金属离子浓度来改善精液质量。本研究的目的是调查用印度人参进行为期3个月的治疗对不育男性精子凋亡和细胞内活性氧（ROS）浓度的影响。在治疗前后，测量精子凋亡、细胞内ROS浓度以及精浆中金属离子铜、锌、铁和金的浓度。通过使用流式细胞术检测膜联蛋白-V结合和碘化丙啶摄取来分析凋亡和坏死细胞分布。通过荧光强度测量ROS生成，并通过原子吸收分光光度法分析金属离子。结果表明，在治疗前，与对照组相比，所有不育男性组的凋亡和细胞内ROS浓度均显著更高。同样，精浆中必需金属离子Cu(2+)、Zn(2+)、Fe(2+)和Au(2+)的浓度较低。用印度人参治疗显著降低了不育受试者的凋亡和ROS浓度，并提高了金属离子浓度。得出的结论是，印度人参通过降低氧化应激和细胞死亡并提高必需金属离子浓度来改善精液质量。
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