Castiglione R, Salemi M, Vicari L O, Vicari E
Section of Endocrinology and Andrology, Department of Medical and Pediatric Science, International Master in Experimental and Clinical Medicine and Cellular Physiopathology, University of Catania, Catania, Italy.
Andrologia. 2014 Dec;46(10):1148-55. doi: 10.1111/and.12207. Epub 2013 Dec 16.
Changes in levels of oxidative damage products in semen and their relationship to seminal fluid viscosity (SFV) have recently received increasing research interest. We analysed whether SFV was associated with ROS generation, levels of cytokines TNF-alpha (TNF-α), IL-6 and IL-10 and seminal leucocyte concentration, and whether ROS production was related to the extent of infections/inflammations at one (prostatitis) or two (prostato-vesiculitis) male accessory glands. We studied 169 infertile patients, with chronic bacterial prostatitis (PR, n = 74) and/or bilateral prostato-vesiculitis (PV, n = 95), as diagnosed by the ultrasound (US) criteria. Healthy fertile men (n = 42) served as controls. In the PV patient group, SFV, semen characteristics and ROS production had median values that were significantly higher than those found in PR patients and controls, although other sperm variables had values significantly lower than those found in PR patients or controls. In PV infertile patients, ROS generation and pro-inflammatory cytokines levels were higher than those found in PR infertile patients and controls, although seminal IL-10 levels in PV and PR patients were lower than those found in the controls. In PR patients, the levels of SFV were positively related to TNF-α (r = 0.67; P < 0.01), fMLP-stimulated ROS production in the 45% Percoll fraction (r = 0.687, P < 0.01) and the 90% Percoll fraction in basal condition (r = 0.695, P < 0.01), and after fMLP-stimulation (r = 0.688, P < 0.01). Thus, our data indicated that seminal hyperviscosity is associated with increased oxidative stress in infertile men and increased pro-inflammatory interleukins in patients with male accessory gland infection, more when the infection was extended to the seminal vesicles.
精液中氧化损伤产物水平的变化及其与精液粘度（SFV）的关系最近受到了越来越多的研究关注。我们分析了SFV是否与活性氧（ROS）生成、细胞因子肿瘤坏死因子-α（TNF-α）、白细胞介素-6和白细胞介素-10水平以及精液白细胞浓度相关，以及ROS产生是否与一个（前列腺炎）或两个（前列腺精囊炎）男性附属腺的感染/炎症程度有关。我们研究了169例不育患者，根据超声（US）标准诊断为慢性细菌性前列腺炎（PR，n = 74）和/或双侧前列腺精囊炎（PV，n = 95）。健康有生育能力的男性（n = 42）作为对照。在PV患者组中，SFV、精液特征和ROS产生的中位数显著高于PR患者和对照组，尽管其他精子变量的值显著低于PR患者或对照组。在PV不育患者中，ROS生成和促炎细胞因子水平高于PR不育患者和对照组，尽管PV和PR患者的精液白细胞介素-10水平低于对照组。在PR患者中，SFV水平与TNF-α呈正相关（r = 0.67；P < 0.01），在45% Percoll组分中fMLP刺激后的ROS产生（r = 0.687，P < 0.01）以及基础条件下90% Percoll组分中的ROS产生（r = 0.695，P < 0.01）和fMLP刺激后（r = 0.688，P < 0.01）。因此，我们的数据表明，精液高粘度与不育男性氧化应激增加以及男性附属腺感染患者促炎白细胞介素增加有关，当感染扩展到精囊时更为明显。
