Laboratory of Developmental Genetics, Department of Human Genetics, Katholieke Universiteit Leuven, Leuven, Belgium.
Development. 2011 Apr;138(8):1595-605. doi: 10.1242/dev.052787. Epub 2011 Mar 9.
The spatiotemporal integration of adhesion and signaling during neuritogenesis is an important prerequisite for the establishment of neuronal networks in the developing brain. In this study, we describe the role of the L1-type CAM Neuroglian protein (NRG) in different steps of Drosophila mushroom body (MB) neuron axonogenesis. Selective axon bundling in the peduncle requires both the extracellular and the intracellular domain of NRG. We uncover a novel role for the ZO-1 homolog Polychaetoid (PYD) in axon branching and in sister branch outgrowth and guidance downstream of the neuron-specific isoform NRG-180. Furthermore, genetic analyses show that the role of NRG in different aspects of MB axonal development not only involves PYD, but also TRIO, SEMA-1A and RAC1.
神经发生过程中黏附和信号的时空整合是大脑发育过程中建立神经元网络的重要前提。在这项研究中，我们描述了 L1 型细胞黏附分子神经生长因子受体相关蛋白（NRG）在果蝇蘑菇体（MB）神经元轴突发生的不同步骤中的作用。在柄部选择性轴突捆绑需要 NRG 的细胞外和细胞内结构域。我们揭示了 ZO-1 同源物多毛类蛋白（PYD）在神经元特异性同工型 NRG-180 下游的轴突分支以及姐妹分支延伸和导向中的新作用。此外，遗传分析表明，NRG 在 MB 轴突发育的不同方面的作用不仅涉及 PYD，还涉及 TRIO、SEMA-1A 和 RAC1。
