Mehrabadi Jalil Fallah, Sirous Mehrandokht, Daryani Naser Ebrahimi, Eshraghi Saeed, Akbari Bahram, Shirazi Mohammad Hasan
MARS bioinformatics Institute, Tehran, Iran.
J Infect Dev Ctries. 2011 Mar 2;5(2):88-93. doi: 10.3855/jidc.1187.
Metronidazole is a significant antibiotic used for eradication of Helicobacter pylori infections and it is of notice that metronidazole-resistant clinical isolates have been found in high rates worldwide. While the RND family of efflux pumps plays a central role in drug resistance among Gram-negative bacteria, this is questionable for H. pylori.
To understand whether TolC homologues of RND pumps contribute to metronidazole resistance in H. pylori isolates, expression of four TolC homologous genes of five resistant clinical isolates exposed to varying concentrations of metronidazole were evaluated by RT-PCR and transcriptional analysis.
The results indicate that excess amounts of metronidazole are able to increase the expression level of these genes at the transcriptional stage.
Therefore, it may be hypothesized that use of metronidazole in H. pyori infection can induce metronidazole resistance. Furthermore, the RND family of efflux pumps may contribute to metronidazole resistance in clinical isolates of H. pylori.
甲硝唑是一种用于根除幽门螺杆菌感染的重要抗生素，值得注意的是，在全球范围内已发现高比例的耐甲硝唑临床分离株。虽然RND家族外排泵在革兰氏阴性菌的耐药性中起核心作用，但对于幽门螺杆菌来说情况存疑。
为了解RND泵的TolC同源物是否有助于幽门螺杆菌分离株对甲硝唑产生耐药性，通过RT-PCR和转录分析评估了5株耐甲硝唑临床分离株在暴露于不同浓度甲硝唑时4个TolC同源基因的表达情况。
结果表明，过量的甲硝唑能够在转录阶段增加这些基因的表达水平。
因此，可以推测在幽门螺杆菌感染中使用甲硝唑可能会诱导产生甲硝唑耐药性。此外，RND家族外排泵可能在幽门螺杆菌临床分离株的甲硝唑耐药性中起作用。
