Stepan-Sarkissian G
Wolfson Institute of Biotechnology, Sheffield, UK.
Methods Mol Biol. 1990;6:1-12. doi: 10.1385/0-89603-161-6:1.
This chapter deals with the selection of growth media for the induction of callus and initiation of liquid suspension cultures from plants. Although the first attempts at initiating cultures of plant cells were made by the German botanist G. Haberlandt at the turn of this century, it has only been during the last three decades that rapid developments in plant cell, tissue, and organ culture have occurred. Since extensive work with microbial cultures was by then already underway, the first medium formulations used for plant culture work were inevitably based on experience of microbes. These media contained several distinct classes of compounds:
本章论述了用于诱导愈伤组织以及从植物起始液体悬浮培养物的生长培养基的选择。虽然在本世纪之交德国植物学家G.哈伯兰特就首次尝试起始植物细胞培养，但直到最近三十年植物细胞、组织和器官培养才得到迅速发展。由于那时对微生物培养的广泛研究已经在进行，用于植物培养工作的首批培养基配方不可避免地是基于对微生物的经验。这些培养基含有几类不同的化合物：
