Dix P J
St.Patrick's College, Kildare, Republic of Ireland.
Methods Mol Biol. 1990;6:455-65. doi: 10.1385/0-89603-161-6:455.
Mutants resistant to chemicals that inhibit growth (antimetabolites) are the most readily selected in plant cell cultures. A number of such mutants have been isolated, with resistance to amino acids and their analogs, base analogs, toxins from pathogenic microorganisms, herbicides, salts, and antibiotics. The mutants can be used for fundamental investigations into cellular metabolism, as markers for plant genetic manipulation, and in efforts to improve crop tolerance to diseases and agrochemicals. Progress in mutant selection has been reviewed extensively in recent years (1-4). Detailed considerations of the technical aspects of in vitro selection are available (1), and some protocols have been published (4-7).
对抑制生长的化学物质（抗代谢物）具有抗性的突变体是植物细胞培养中最容易筛选出来的。已经分离出许多这样的突变体，它们对氨基酸及其类似物、碱基类似物、病原微生物毒素、除草剂、盐类和抗生素具有抗性。这些突变体可用于细胞代谢的基础研究、作为植物基因操作的标记，以及用于提高作物对疾病和农用化学品的耐受性。近年来，突变体筛选方面的进展已有广泛综述(1 - 4)。关于体外筛选技术方面的详细论述也有可得(1)，并且一些实验方案已经发表(4 - 7)。
