IRIS-International Research Institute of Stavanger, Stavanger, Norway.
J Toxicol Environ Health A. 2011;74(7-9):569-81. doi: 10.1080/15287394.2011.550565.
The measurement of low-concentration alkylphenol (AP) exposure in fish is relevant in connection with monitoring and risk assessment of offshore oil industry produced water (PW) discharges. Detection of AP markers in fish bile offers significantly greater sensitivity than detection of AP in tissues such as liver. Recent studies revealed that gas chromatography-mass spectrometry in electron ionization mode (GC-EI-MS) enabled a selective and sensitive analytical detection of PW AP in mixtures with unknown composition. A procedure consisting of enzymatic deconjugation of metabolites in fish bile followed by derivatization with bis(trimethylsilyl)trifluoroacetamide and then separation and quantification of derivatized AP using GC-EI-MS is presented. The use of this procedure as a possible recommended approach for assessment and biomonitoring of AP contamination in fish populations living down-current from offshore oil production fields is presented.
测定鱼类中的低浓度烷基酚（AP）暴露水平与监测和评估近海石油工业生产污水（PW）排放的风险有关。在鱼类胆汁中检测 AP 标志物的灵敏度明显高于在肝脏等组织中检测 AP 的灵敏度。最近的研究表明，电子电离模式下的气相色谱-质谱法（GC-EI-MS）能够选择性和灵敏地分析检测混合物中的 PWAP，而无需事先知道其组成。本研究提出了一种由酶促法使鱼胆汁中的代谢物去共轭化，然后用双（三甲基硅基）三氟乙酰胺衍生化，再用 GC-EI-MS 对衍生化的 AP 进行分离和定量的方法。本研究还提出了将此方法作为评估和生物监测生活在近海石油生产场下游鱼类种群中 AP 污染的一种推荐方法。
