Vyskochil F, Magazanik L G
Zh Evol Biokhim Fiziol. 1978 Sep-Oct;14(5):448-52.
The spontaneous excitatory junctional currents (e. j. c.'s) were recorded intracellulary using voltage clamp technique in experiments performed on the isolated cutaneo-muscle bag of larvae, early pupae stadium and adult fly Drosophila melanogaster. The fast miniature e. j. c.'s with mean amplitude 0.41 nA, rise time 1.60 ms and half-decay time 3.11 ms were most frequently observed. Besides these, slow miniature (half--decay time 7--20 ms) and fast giant (amplitude 12--15 nA) were sometimes present. It was found that the time course of fast e. j. c.'s is prolonged with hyperpolarisation and with the decrease in temperature. Some drugs which effectively modify the time course of end-plate currents at the cholinergic junctions (atropine, scopolamine, lidocaine, serotonin, QX-222 and ethanol) were tested. Except for ethanol, none of these drugs when added to the muscle bath affected either the amplitude or the time course of e. j. c.'s. The properties of e. j. c.'s in different neuro-muscular junctions of vertebrates and insects are discussed.
在对黑腹果蝇幼虫、早蛹期和成虫的离体皮肌袋进行的实验中，使用电压钳技术在细胞内记录自发兴奋性突触后电流（e.j.c.）。最常观察到的是快速微小e.j.c.，其平均幅度为0.41 nA，上升时间为1.60 ms，半衰减时间为3.11 ms。除此之外，有时还会出现缓慢微小的（半衰减时间为7 - 20 ms）和快速巨大的（幅度为12 - 15 nA）。研究发现，快速e.j.c.的时间进程会随着超极化和温度降低而延长。测试了一些能有效改变胆碱能突触终板电流时间进程的药物（阿托品、东莨菪碱、利多卡因、血清素、QX - 222和乙醇）。除了乙醇外，将这些药物添加到肌肉浴中均不会影响e.j.c.的幅度或时间进程。文中讨论了脊椎动物和昆虫不同神经肌肉接头处e.j.c.的特性。
