Department of Experimental and Health Sciences, Pompeu Fabra University, Barcelona Biomedical Research Park, Barcelona, Spain.
J Pept Sci. 2011 Apr;17(4):247-51. doi: 10.1002/psc.1310. Epub 2010 Nov 29.
Dendrimeric platforms such as MAPs can be synthesized either entirely by solid-phase methods (SPPS, direct approach) or by conjugation in solution of preformed, SPPS-made building blocks (indirect approach). Although MAPs and MAP-like constructs have been extensively and successfully used for various biological (mainly immunological) applications, experimental reports are most often lacking in chemical detail about their preparation and characterization. Here, we provide complete accounts of the synthesis and analytical documentation of MAPs and similar dendrimers by either all-SPPS (direct) or chemoselective thioether ligation (indirect) methods. We have chosen as model epitopes a 24-residue sequence of the ectodomain of protein M2 from influenza virus (M2e), which is found to be a rather challenging peptide epitope, and a far more manageable, shortened (12-residue) version of the same peptide. The advantages and shortcomings of both direct and indirect methods are discussed.
树状聚合物平台，如 MAPs，可以通过固相法（SPPS，直接法）或通过预成型、SPPS 制造的构建块在溶液中的共轭（间接法）来合成。尽管 MAPs 和类似的树突状聚合物已被广泛成功地用于各种生物学（主要是免疫学）应用，但实验报告通常缺乏有关其制备和表征的化学细节。在这里，我们通过全固相法（直接法）或化学选择性硫醚连接（间接法）方法，提供了 MAPs 和类似树突状聚合物的合成和分析文件的完整说明。我们选择了流感病毒蛋白 M2 的外域的 24 个残基序列作为模型表位（M2e），它被发现是一个相当具有挑战性的肽表位，以及相同肽的更易于管理的缩短（12 个残基）版本。讨论了直接和间接方法的优缺点。
