Department of Biochemistry, Nobel Medical College Teaching Hospital, Biratnagar, Morang, Nepal.
Department of Medicine, Nobel Medical College Teaching Hospital, Biratnagar, Morang, Nepal.
JNMA J Nepal Med Assoc. 2022 Feb 15;60(246):155-159. doi: 10.31729/jnma.5253.
The cases of dengue fever have been reported more frequently in Nepal these days. The aim of this study was to find the prevalence of seropositivity among blood samples drawn from suspected dengue cases at a tertiary care centre of Nepal.
A descriptive cross-sectional study was conducted at a tertiary care hospital from 1st June 2017 to 31st October 2018 after getting approval from the Institutional Review Committee (Reference number: 23/2016). A total of 537 suspected dengue patients were selected for the study using convenience sampling. These dengue positive sera were assayed for their reactivity with Immunoglobulin M and Immunoglobulin G present in sera and synthetic peptides of dengue virus by enzyme linked immunosorbent assay. Data was entered and analysed in Microsoft Excel 2016. Point estimate at 95% Confidence Interval was calculated along with frequency, percentage, mean and standard deviation.
Among 537 suspected dengue cases, the seropositivity for dengue was found in 124 (23.09%) (19.52-26.65 at 95% Confidence Interval) of the serum.
The present study revealed that dengue was more prevalent in our setting compared to similar studies. All the synthetic peptides showed reactivity with dengue-positive sera with maximum reactivity shown by RR2 peptide. In dengue-positive sera, RR2 peptide of dengue virus identified more Immunoglobulin M than Immunoglobulin G.
如今，尼泊尔的登革热病例报告越来越频繁。本研究旨在调查尼泊尔一家三级护理中心疑似登革热病例的血清阳性率。
本研究为描述性横断面研究，于 2017 年 6 月 1 日至 2018 年 10 月 31 日在获得机构审查委员会批准（参考号：23/2016）后在一家三级医院进行。采用便利抽样法，共选取 537 例疑似登革热患者进行研究。采用酶联免疫吸附试验检测登革热阳性血清中与血清中存在的免疫球蛋白 M 和免疫球蛋白 G 以及登革热病毒合成肽的反应性。数据在 Microsoft Excel 2016 中输入和分析。计算了 95%置信区间的点估计值以及频率、百分比、均值和标准差。
在 537 例疑似登革热病例中，血清登革热血清阳性率为 124 例（23.09%）（95%置信区间为 19.52-26.65）。
本研究表明，与类似研究相比，登革热在本研究中更为普遍。所有合成肽均与登革热阳性血清反应，RR2 肽反应性最强。在登革热阳性血清中，登革热病毒的 RR2 肽识别出更多的免疫球蛋白 M 而不是免疫球蛋白 G。
