Xu Wen, Hu Rong, Fan Erzhong, Han Demin
Department of Otorhinolaryngology-Head Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
Ann Otol Rhinol Laryngol. 2011 Feb;120(2):123-30. doi: 10.1177/000348941112000209.
We sought to characterize the changes in the extracellular matrix (ECM) of the lamina propria following the implantation of autologous adipose-derived mesenchymal stem cells (ADSCs) in composite scaffolds in a rabbit vocal fold wound model.
The ADSCs were co-cultured with collagen or hyaluronic acid gel. Each vocal fold was injured by localized resection and injected with ADSC complexes, ADSCs, or scaffolds or left untreated. From 15 days to 12 months, the vocal fold shape and the characteristics of the ECM components were analyzed.
With implantation of the ADSC complexes, the collagen content was significantly increased and had a disorderly distribution at 3 months. Subsequently, it began to decrease and reached close to normal by 12 months. The hyaluronic acid content was increased at 40 days, but it was reduced to normal levels and was limited to the superficial and middle layers of the lamina propria over the following 12 months. Fibronectin continued to be scattered in the lamina propria, at peak levels at 40 days, and then decreased over the following 12 months to reach near-normal levels. At 12 months, the vocal folds had a normal surface.
ADSC complexes can play a facilitatory role in vocal fold regeneration, regulating the generation and orderly distribution of ECM.
我们试图在兔声带伤口模型中，对自体脂肪来源的间充质干细胞（ADSCs）植入复合支架后固有层细胞外基质（ECM）的变化进行表征。
将ADSCs与胶原蛋白或透明质酸凝胶共培养。通过局部切除对每条声带造成损伤，并注射ADSC复合物、ADSCs或支架，或不进行处理。在15天至12个月的时间里，分析声带形状和ECM成分的特征。
植入ADSC复合物后，3个月时胶原蛋白含量显著增加且分布紊乱。随后，其开始下降，到12个月时接近正常水平。透明质酸含量在40天时增加，但在接下来的12个月里降至正常水平，并局限于固有层的浅层和中层。纤连蛋白继续分散在固有层中，在40天时达到峰值水平，然后在接下来的12个月里下降至接近正常水平。在12个月时，声带表面正常。
ADSC复合物可在声带再生中发挥促进作用，调节ECM的生成和有序分布。
