Valerie Angelou, Vassiliki Kalodimou, Irini Messini, Nikolaos Psychalakis, Karampela Eleftheria, Apostolos Papalois
Phoniatrics and Applied Otorhinolaryngology Center, Amalias Avenue 42, 10542 Athens, Greece.
Department of Flow Cytometry-Research & Regenerative Medicine, IASO Maternity Hospital, Kifissias Avenue 37-39, Maroussi, 15123 Athens, Greece.
Stem Cells Int. 2016;2016:9010279. doi: 10.1155/2016/9010279. Epub 2016 Jan 6.
Background. The aim of the study was to assess the histological effects of autologous infusion of adipose-derived stem cells (ADSC) on a chronic vocal fold scar in a rabbit model as compared to an untreated scar as well as in injection of hyaluronic acid. Study Design. Animal experiment. Method. We used 74 New Zealand rabbits. Sixteen of them were used as control/normal group. We created a bilateral vocal fold wound in the remaining 58 rabbits. After 18 months we separated our population into three groups. The first group served as control/scarred group. The second one was injected with hyaluronic acid in the vocal folds, and the third received an autologous adipose-derived stem cell infusion in the scarred vocal folds (ADSC group). We measured the variation of thickness of the lamina propria of the vocal folds and analyzed histopathologic changes in each group after three months. Results. The thickness of the lamina propria was significantly reduced in the group that received the ADSC injection, as compared to the normal/scarred group. The collagen deposition, the hyaluronic acid, the elastin levels, and the organization of elastic fibers tend to return to normal after the injection of ADSC. Conclusions. Autologous injection of adipose-derived stem cells on a vocal fold chronic scar enhanced the healing of the vocal folds and the reduction of the scar tissue, even when compared to other treatments.
背景。本研究的目的是评估自体输注脂肪干细胞（ADSC）对兔模型中慢性声带瘢痕的组织学影响，并与未治疗的瘢痕以及注射透明质酸的情况进行比较。研究设计。动物实验。方法。我们使用了74只新西兰兔。其中16只作为对照/正常组。我们在其余58只兔中制造了双侧声带伤口。18个月后，我们将这些兔子分为三组。第一组作为对照/瘢痕组。第二组在声带中注射透明质酸，第三组在瘢痕化的声带中接受自体脂肪干细胞输注（ADSC组）。三个月后，我们测量了声带固有层厚度的变化，并分析了每组的组织病理学变化。结果。与正常/瘢痕组相比，接受ADSC注射的组中固有层厚度显著降低。注射ADSC后，胶原蛋白沉积、透明质酸、弹性蛋白水平以及弹性纤维的组织结构趋向于恢复正常。结论。即使与其他治疗方法相比，自体注射脂肪干细胞到声带慢性瘢痕中也能促进声带愈合并减少瘢痕组织。
