Eugster Philippe J, Guillarme Davy, Rudaz Serge, Veuthey Jean-Luc, Carrupt Pierre-Alain, Wolfender Jean-Luc
School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, 30, Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
J AOAC Int. 2011 Jan-Feb;94(1):51-70.
Ultra high pressure liquid chromatography (UHPLC) systems operating at very high pressures and using sub-2 microm packing columns have allowed a remarkable decrease in analysis time and increase in peak capacity, sensitivity, and reproducibility compared to conventional HPLC. This technology has rapidly been widely accepted by the analytical community and is being gradually applied to various fields of plant analysis such as QC, profiling and fingerprinting, dereplication, and metabolomics. For many applications, an important improvement of the overall performances has been reported. In this review, the basic principles of UHPLC are summarized, and practical information on the type of columns used and phase chemistry available is provided. An overview of the latest applications to natural product analysis in complex mixtures is given, and the potential and limitations as well as some new trends in the development of UHPLC are discussed.
与传统高效液相色谱（HPLC）相比，超高压液相色谱（UHPLC）系统在极高压力下运行，并使用粒径小于2微米的填充柱，可显著缩短分析时间，提高峰容量、灵敏度和重现性。这项技术已迅速被分析界广泛接受，并正逐渐应用于植物分析的各个领域，如质量控制、图谱分析和指纹识别、去重复和代谢组学。对于许多应用，已有报道称整体性能有了重要改进。在本综述中，总结了UHPLC的基本原理，并提供了所用柱类型和可用相化学的实用信息。概述了其在复杂混合物天然产物分析中的最新应用，并讨论了UHPLC发展的潜力、局限性以及一些新趋势。
