Gujarat Cancer and Research Institute, Asarwa, Ahmedabad 380 016, Gujarat, India.
Neoplasma. 2011;58(3):217-26. doi: 10.4149/neo_2011_03_217.
The purpose of the present study was to identify site-specific prognostic biomarkers in patients with oral squamous cell carcinoma (OSCC). For this purpose, Epidermal growth factor receptor (EGFR), Stat3, H-ras, c-myc, p53, cyclin D1, p16, Rb and Bcl-2 were localized immunohistochemically in buccal mucosa carcinoma (n=74) and tongue carcinoma (n=61) patients. Expression of markers was compared between buccal mucosa and tongue carcinoma and assessed for their prognostic value in site-specific manner. On comparison, only cyclin D1 showed significant difference in expression with higher accumulation in tongue tumors (r=+0.177, p=0.039). Moreover, univariate survival analysis showed that in buccal mucosa patients, loss of p16 and overexpression of H-ras were significant prognosticators for relapse-free survival (RFS) and overall survival (OS), respectively. However, in Cox multivariate analysis, they lost their significance after adjusting for significant clinicopathological parameters. On the other hand, in tongue cancer patients, Cox multivariate analysis showed that for RFS, Stat3 and c-myc, and for OS, Stat3, Bcl-2 and p53 were significant prognosticators after adjusting for significant confounding factors. Our findings indicated that buccal mucosa and tongue carcinoma exhibit different biological behavior which is reflected in prognosis. Therefore, this approach might be helpful to precisely identify patients for more effectively tailored treatment strategy.
本研究旨在确定口腔鳞状细胞癌（OSCC）患者的特定部位预后生物标志物。为此，我们采用免疫组织化学方法对 74 例颊黏膜癌和 61 例舌癌患者的表皮生长因子受体（EGFR）、Stat3、H-ras、c-myc、p53、cyclin D1、p16、Rb 和 Bcl-2 进行了定位。比较了颊黏膜和舌癌中标记物的表达，并评估了它们在特定部位的预后价值。结果表明，只有 cyclin D1 的表达有显著差异，舌肿瘤中 cyclin D1 的表达更高（r=+0.177，p=0.039）。此外，单因素生存分析显示，在颊黏膜患者中，p16 缺失和 H-ras 过表达分别是无复发生存（RFS）和总生存（OS）的显著预后因素。然而，在 Cox 多因素分析中，在调整了显著的临床病理参数后，它们失去了意义。另一方面，在舌癌患者中，Cox 多因素分析显示，对于 RFS，Stat3 和 c-myc，以及对于 OS，Stat3、Bcl-2 和 p53 是调整了显著混杂因素后的显著预后因素。我们的研究结果表明，颊黏膜和舌癌表现出不同的生物学行为，这反映在预后上。因此，这种方法可能有助于更准确地识别患者，以便制定更有效的个体化治疗策略。
