Krishna Akhilesh, Singh Shraddha, Singh Vineeta, Kumar Vijay, Singh Uma Shankar, Sankhwar Satya Narayan
Department of Physiology, King George's Medical University, Lucknow, Uttar Pradesh, India.
Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh, India.
J Oral Maxillofac Pathol. 2018 Jan-Apr;22(1):65-72. doi: 10.4103/jomfp.JOMFP_246_17.
Harvey-Ras (H-Ras) is an important guanosine triphosphatase protein for the regulation of cellular growth and survival. Altered Ras signaling has been observed in different types of cancer either by gene amplification and/or mutation. The H-Ras oncogene mutations are well reported, but expression of the H-Ras gene is still unknown.
This study aimed to examine both protein and messenger-RNA (mRNA) expressions of H-Ras in oral squamous cell carcinoma (OSCC) and analyzed the association with risk habits and the clinicopathological profile of cases.
A total of 65 tissue specimens of OSCC (case group) and equal number of normal tissues (control group) were included in this study. H-Ras protein and mRNA expressions were analyzed using immunohistochemical and quantitative real time-polymerase chain reaction techniques, respectively.
The H-Ras protein was significantly overexpressed in the oral carcinoma group compared to the normal group ( = 0.03). Most of the OSCC cases showed positive staining with moderate expression, while negative and moderate staining was high in the control group. The majority of H-Ras positive cases were found in individuals with multiple risk habits including tobacco chewing. The risk of H-Ras positivity was 1.46 times higher in smokers than non-smokers. H-Ras positivity increased in cases affected with buccal mucosa site and higher grade of carcinoma. Relative mRNA level of H-Ras was significantly elevated in oral carcinoma as compared with the control group ( ≤ 0.001). Protein and mRNA levels of H-Ras in case group was poorly correlated.
H-Ras oncogene expression was markedly higher in oral carcinoma, and it can be a prognostic marker and target for an effective molecular therapy.
哈维 - 拉斯（H - Ras）是一种重要的鸟苷三磷酸酶蛋白，用于调节细胞生长和存活。在不同类型的癌症中，通过基因扩增和/或突变观察到Ras信号通路发生改变。H - Ras癌基因突变已有充分报道，但H - Ras基因的表达情况仍不清楚。
本研究旨在检测口腔鳞状细胞癌（OSCC）中H - Ras的蛋白质和信使核糖核酸（mRNA）表达，并分析其与风险习惯及病例临床病理特征的关系。
本研究共纳入65例OSCC组织标本（病例组）和数量相等的正常组织（对照组）。分别采用免疫组织化学和定量实时聚合酶链反应技术分析H - Ras蛋白和mRNA表达。
与正常组相比，口腔癌组中H - Ras蛋白显著过表达（P = 0.03）。大多数OSCC病例显示中度表达的阳性染色，而对照组中阴性和中度染色比例较高。大多数H - Ras阳性病例见于有多种风险习惯的个体，包括咀嚼烟草。吸烟者中H - Ras阳性的风险是非吸烟者的1.46倍。颊黏膜部位受累且癌症分级较高的病例中H - Ras阳性率增加。与对照组相比，口腔癌中H - Ras的相对mRNA水平显著升高（P≤0.001）。病例组中H - Ras的蛋白和mRNA水平相关性较差。
H - Ras癌基因在口腔癌中的表达明显更高，它可以作为一种预后标志物和有效分子治疗的靶点。
