MIRA Institute for Biomedical Engineering and Technical Medicine, Faculty of Science and Technology, University of Twente, 7500 AE Enschede, The Netherlands.
Annu Rev Anal Chem (Palo Alto Calif). 2011;4:227-49. doi: 10.1146/annurev-anchem-061010-114024.
Nuclear magnetic resonance (NMR) spectroscopy is one of the most information-rich analytical techniques available. However, it is also inherently insensitive, and this drawback precludes the application of NMR spectroscopy to mass- and volume-limited samples. We review a particular approach to increase the sensitivity of NMR experiments, namely the use of miniaturized coils. When the size of the coil is reduced, the sample volume can be brought down to the nanoliter range. We compare the main coil geometries (solenoidal, planar, and microslot/stripline) and discuss their applications to the analysis of mass-limited samples. We also provide an overview of the hyphenation of microcoil NMR spectroscopy to separation techniques and of the integration with lab-on-a-chip devices and microreactors.
核磁共振（NMR）光谱学是最具信息丰富的分析技术之一。然而，它本身也很不灵敏，这一缺点使得 NMR 光谱学无法应用于质量和体积有限的样品。我们回顾了一种提高 NMR 实验灵敏度的特殊方法，即使用微型线圈。当线圈的尺寸减小时，样品的体积可以降低到纳升级。我们比较了主要的线圈几何形状（螺线管、平面和微槽/微带），并讨论了它们在分析质量有限的样品中的应用。我们还概述了微线圈 NMR 光谱学与分离技术的联用，以及与微流控芯片装置和微反应器的集成。
