Department of Biotechnology, Central Leather Research Institute (Council of Scientific and Industrial Research), Adyar, Chennai 20, Tamil Nadu, India.
Int J Pharm. 2011 May 30;410(1-2):145-52. doi: 10.1016/j.ijpharm.2011.02.065. Epub 2011 Mar 29.
Growth factors are essential in cellular signaling for migration, proliferation, differentiation and maturation. Sustainable delivery of therapeutic as well as functional proteins is largely required in the pharmacological and regenerative medicine. Here we have prepared chitosan nanoparticles (CNP) and incorporated growth factors such as epidermal growth factor (EGF) and fibroblast growth factor (FGF), either individually or in combination, which could ultimately be impregnated into engineered tissue construct. CNP was characterized by Fourier transform infrared (FTIR) spectroscopy, Zeta sizer and high resolution transmission electron microscope (HRTEM). The particles were in the size range of 50-100 nm with round and flat shape. The release kinetics of both EGF and FGF incorporated CNP showed the release of growth factors in a sustained manner. Growth factors incorporated nanoparticles did not show any toxicity against fibroblasts up to 4 mg/ml culture medium. Increased proliferation of fibroblasts in vitro evidenced the delivery of growth factors from CNP for cellular signaling. Western blotting results also revealed the poor inflammatory response showing less expression of proinflammatory cytokines such as IL-6 and TNFα in the macrophage cell line J774 A-1.
生长因子在细胞信号转导中对于迁移、增殖、分化和成熟是必不可少的。在药理学和再生医学中，大量需要可持续地递呈治疗性和功能性蛋白质。在这里，我们制备了壳聚糖纳米颗粒（CNP），并将生长因子（如表皮生长因子（EGF）和成纤维细胞生长因子（FGF））单独或组合掺入其中，最终可以将其浸渍到工程化组织构建体中。CNP 通过傅里叶变换红外（FTIR）光谱、Zeta 粒径仪和高分辨率透射电子显微镜（HRTEM）进行了表征。颗粒的尺寸范围为 50-100nm，呈圆形和平坦形状。载有 EGF 和 FGF 的 CNP 的释放动力学显示出生长因子以持续的方式释放。在 4mg/ml 培养基中，掺入纳米颗粒的生长因子对成纤维细胞没有显示出任何毒性。体外成纤维细胞增殖增加证明了 CNP 可用于细胞信号转导来递呈生长因子。Western 印迹结果还表明，巨噬细胞系 J774A-1 中促炎细胞因子（如 IL-6 和 TNFα）的表达较少，表明炎症反应较差。
