Departamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, 28040, Madrid, Spain.
Acta Biomater. 2011 Jul;7(7):2977-85. doi: 10.1016/j.actbio.2011.03.005. Epub 2011 Mar 22.
In this manuscript in vitro bacterial adhesion assays using Escherichia coli on different SBA-15 nanostructured ceramics have been performed. For this purpose pure silica, NH(2) or COOH monofunctionalized, and NH(2)/COOH bifunctionalized SBA-15 mesoporous materials have been used. Material characterization reveals that both NH(2)/COOH and NH(2) functionalized SBA-15 materials exhibit a zwitterionic character due to the presence of -NH(3)(+)/COO(-) or -NH(3)(+)/SiO(-) moieties, respectively. In vitro adhesion assays have been carried out at the pH at which the zwitterionic nature of both of these samples is preserved, i.e. pH 5.5. The results show that the presence of both positive and negative moieties with an overall neutral charge leads to reduced E. coli adhesiveness. In vitro tests with cultured human Saos-2 osteoblasts have been carried out to evaluate the biocompatibility of the different materials at the physiological pH of 7.4. The results demonstrate that all materials exhibit good biocompatibility, with Saos-2 osteoblasts adhering, proliferating and maintaining their morphological and functional characteristics. This novel family of zwitterionic mesoporous materials opens up promising expectations in diverse biomedical applications, such as preventing some side-effects associated with bone implant infections.
在本手稿中，使用大肠杆菌在不同 SBA-15 纳米结构陶瓷上进行了体外细菌粘附实验。为此，使用了纯二氧化硅、NH(2)或 COOH 单官能化以及 NH(2)/COOH 双官能化 SBA-15 介孔材料。材料特性表明，由于存在-NH(3)(+)/COO(-)或-NH(3)(+)/SiO(-)部分，NH(2)/COOH 和 NH(2)官能化 SBA-15 材料都具有两性离子特性。体外粘附实验在保持这两种样品两性离子特性的 pH 值（即 pH 5.5）下进行。结果表明，存在带正电荷和带负电荷的基团，且总电荷为中性，导致大肠杆菌的粘附性降低。在生理 pH 值为 7.4 的条件下，用培养的人 Saos-2 成骨细胞进行了体外测试，以评估不同材料的生物相容性。结果表明，所有材料都表现出良好的生物相容性，Saos-2 成骨细胞能够附着、增殖并保持其形态和功能特征。这种新型两性离子介孔材料为预防与骨植入物感染相关的一些副作用等各种生物医学应用提供了广阔的前景。
