Department of Anesthesiology, Changhai Hospital, Shanghai, China.
J Surg Res. 2011 May 15;167(2):e339-44. doi: 10.1016/j.jss.2010.11.005. Epub 2010 Dec 2.
Recently it has been demonstrated that hydrogen, as a novel antioxidant, can selectively reduce hydroxyl radicals (·OH) and peroxynitrite anion (ONOO-) in vitro and exert therapeutic antioxidant activity in many diseases. This study was designed to investigate the effect of hydrogen-rich saline on renal ischemia/reperfusion (I/R) injury in rats.
A rat model of renal I/R injury was induced by 45-min occlusion of the bilateral renal pedicles and 24-h reperfusion. Physiologic saline, hydrogen-rich saline, or nitrogen-rich saline (8 mL/kg) were administered intraperitoneally at 5 min before reperfusion, respectively.
After I/R injury, serum blood urea nitrogen (BUN), creatinine (Cr), tissue malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OhdG), TNF-α, IL-1β, IL-6 levels, and myeloperoxidase (MPO) activity were all increased significantly, while tissue superoxide dismutase (SOD) and catalase (CAT) activities were all decreased significantly. Hydrogen-rich saline reversed these changes and relieved morphological renal injury and I/R-induced apoptosis, while no significant changes were observed in the nitrogen-rich saline-treated group compared with physiologic saline-treated group.
Hydrogen-rich saline is able to attenuate the renal I/R injury, which is possibly by reduction of oxidative stress and inflammation.
最近有研究表明，氢气作为一种新型抗氧化剂，能够在体外选择性还原羟自由基（·OH）和过氧亚硝酸阴离子（ONOO-），并在许多疾病中发挥治疗性抗氧化作用。本研究旨在探讨富氢生理盐水对大鼠肾缺血再灌注（I/R）损伤的影响。
通过双侧肾蒂夹闭 45 分钟和再灌注 24 小时诱导大鼠肾 I/R 损伤模型。在再灌注前 5 分钟分别给予生理盐水、富氢生理盐水或富氮生理盐水（8 mL/kg）腹腔内注射。
I/R 损伤后，血清尿素氮（BUN）、肌酐（Cr）、组织丙二醛（MDA）、8-羟基脱氧鸟苷（8-OhdG）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）水平和髓过氧化物酶（MPO）活性均显著升高，而组织超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性均显著降低。富氢生理盐水逆转了这些变化，缓解了形态学肾损伤和 I/R 诱导的细胞凋亡，而富氮生理盐水处理组与生理盐水处理组相比，无明显变化。
富氢生理盐水能够减轻肾 I/R 损伤，可能是通过降低氧化应激和炎症反应实现的。
