富氢盐水减轻大鼠肠缺血/再灌注诱导的肺损伤。
Hydrogen-rich saline reduces lung injury induced by intestinal ischemia/reperfusion in rats.
作者信息
Mao Yan-Fei, Zheng Xing-Feng, Cai Jian-Mei, You Xin-Min, Deng Xiao-Ming, Zhang John H, Jiang Lai, Sun Xue-Jun
机构信息
Department of Surgical Intensive Care Unit, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, PR China.
出版信息
Biochem Biophys Res Commun. 2009 Apr 17;381(4):602-5. doi: 10.1016/j.bbrc.2009.02.105. Epub 2009 Feb 26.
OBJECTIVE
Hydrogen has been reported to selectively reduce the hydroxyl radical, the most cytotoxic of reactive oxygen species. In this study we investigated the effects of hydrogen-rich saline on the prevention of lung injury induced by intestinal ischemia/reperfusion (I/R) in rats.
METHODS
Male Sprague-Dawley rats (n=30, 200-220g) were divided randomly into three experimental groups: sham operated, intestinal I/R plus saline treatment (5ml/kg, i.v.), and intestinal I/R plus hydrogen-rich saline treatment (5ml/kg, i.v.) groups. Intestinal I/R was produced by 90min of intestinal ischemia followed by a 4h of reperfusion.
RESULTS
Hydrogen-rich saline treatment decreased the neutrophil infiltration, the lipid membrane peroxidation, NF-kappaB activation and the pro-inflammatory cytokine interleukin IL-1beta and TNF-alpha in the lung tissues compared with those in saline-treated rat.
CONCLUSION
Hydrogen-rich saline attenuates lung injury induced by intestinal I/R.
目的
据报道，氢气可选择性地还原羟基自由基，这是活性氧中细胞毒性最强的。在本研究中，我们调查了富氢盐水对预防大鼠肠缺血/再灌注（I/R）诱导的肺损伤的作用。
方法
雄性Sprague-Dawley大鼠（n=30，体重200-220g）被随机分为三个实验组：假手术组、肠I/R加盐水治疗（5ml/kg，静脉注射）组和肠I/R加富氢盐水治疗（5ml/kg，静脉注射）组。通过90分钟的肠缺血，随后4小时的再灌注来制造肠I/R。
结果
与盐水治疗的大鼠相比，富氢盐水治疗降低了肺组织中的中性粒细胞浸润、脂质膜过氧化、NF-κB激活以及促炎细胞因子白细胞介素IL-1β和TNF-α。
结论
富氢盐水减轻了肠I/R诱导的肺损伤。
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