Mao Yan-Fei, Zheng Xing-Feng, Cai Jian-Mei, You Xin-Min, Deng Xiao-Ming, Zhang John H, Jiang Lai, Sun Xue-Jun
Department of Surgical Intensive Care Unit, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, PR China.
Biochem Biophys Res Commun. 2009 Apr 17;381(4):602-5. doi: 10.1016/j.bbrc.2009.02.105. Epub 2009 Feb 26.
Hydrogen has been reported to selectively reduce the hydroxyl radical, the most cytotoxic of reactive oxygen species. In this study we investigated the effects of hydrogen-rich saline on the prevention of lung injury induced by intestinal ischemia/reperfusion (I/R) in rats.
Male Sprague-Dawley rats (n=30, 200-220g) were divided randomly into three experimental groups: sham operated, intestinal I/R plus saline treatment (5ml/kg, i.v.), and intestinal I/R plus hydrogen-rich saline treatment (5ml/kg, i.v.) groups. Intestinal I/R was produced by 90min of intestinal ischemia followed by a 4h of reperfusion.
Hydrogen-rich saline treatment decreased the neutrophil infiltration, the lipid membrane peroxidation, NF-kappaB activation and the pro-inflammatory cytokine interleukin IL-1beta and TNF-alpha in the lung tissues compared with those in saline-treated rat.
Hydrogen-rich saline attenuates lung injury induced by intestinal I/R.
据报道，氢气可选择性地还原羟基自由基，这是活性氧中细胞毒性最强的。在本研究中，我们调查了富氢盐水对预防大鼠肠缺血/再灌注（I/R）诱导的肺损伤的作用。
雄性Sprague-Dawley大鼠（n=30，体重200-220g）被随机分为三个实验组：假手术组、肠I/R加盐水治疗（5ml/kg，静脉注射）组和肠I/R加富氢盐水治疗（5ml/kg，静脉注射）组。通过90分钟的肠缺血，随后4小时的再灌注来制造肠I/R。
与盐水治疗的大鼠相比，富氢盐水治疗降低了肺组织中的中性粒细胞浸润、脂质膜过氧化、NF-κB激活以及促炎细胞因子白细胞介素IL-1β和TNF-α。
富氢盐水减轻了肠I/R诱导的肺损伤。
