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来自黄孢原毛平革菌的内切-1,4-β-木聚糖酶A在毕赤酵母中的异源表达。

Heterologous Expression of Endo-1,4-beta-xylanaseA from Phanerochaete chrysosporium in Pichia pastoris.

作者信息

Huy Nguyen Duc, Thiyagarajan Saravanakumar, Son Yu-Lim, Park Seung-Moon

机构信息

Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea.

出版信息

Mycobiology. 2011 Jun;39(2):121-4. doi: 10.4489/MYCO.2011.39.2.121. Epub 2011 Jun 16.

DOI:10.4489/MYCO.2011.39.2.121
PMID:22783089
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3385102/
Abstract

The cDNA of endo-1,4-β-xylanaseA, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseA is efficiently secreted into the medium at maximum concentrations of 1,946 U/L and 2,496 U/L, respectively.

摘要

从黄孢原毛平革菌中分离得到的内切-1,4-β-木聚糖酶A的cDNA在毕赤酵母中表达。分别使用木聚糖酶A的内在前导肽或酿酒酵母的α-因子信号肽，木聚糖酶A分别以1946 U/L和2496 U/L的最大浓度有效分泌到培养基中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae2a/3385102/fb5d30e8b517/mb-39-121-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae2a/3385102/666f695f7abc/mb-39-121-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae2a/3385102/179cc658eb64/mb-39-121-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae2a/3385102/fb5d30e8b517/mb-39-121-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae2a/3385102/666f695f7abc/mb-39-121-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae2a/3385102/179cc658eb64/mb-39-121-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae2a/3385102/fb5d30e8b517/mb-39-121-g003.jpg

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Expression of endo-1, 4-beta-xylanase from Trichoderma reesei in Pichia pastoris and functional characterization of the produced enzyme.里氏木霉内切-1,4-β-木聚糖酶在毕赤酵母中的表达及所产酶的功能特性研究
BMC Biotechnol. 2009 Jun 16;9:56. doi: 10.1186/1472-6750-9-56.
3
Expression of recombinant proteins in Pichia pastoris.
重组蛋白在毕赤酵母中的表达。
Appl Biochem Biotechnol. 2007 Aug;142(2):105-24. doi: 10.1007/s12010-007-0003-x.
4
Production of recombinant protein in Pichia pastoris by fermentation.通过发酵在毕赤酵母中生产重组蛋白。
Nat Protoc. 2006;1(2):1006-21. doi: 10.1038/nprot.2006.126.
5
Heterologous protein production using the Pichia pastoris expression system.利用巴斯德毕赤酵母表达系统生产异源蛋白。
Yeast. 2005 Mar;22(4):249-70. doi: 10.1002/yea.1208.
6
Xylanases, xylanase families and extremophilic xylanases.木聚糖酶、木聚糖酶家族及嗜极木聚糖酶
FEMS Microbiol Rev. 2005 Jan;29(1):3-23. doi: 10.1016/j.femsre.2004.06.005.
7
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